SMCR7/MID49 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMCR7/MID49 Polyklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (2)

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

50

Rezensionen (1)

Kat-Nr. : 16413-1-AP

Synonyme

MID49, MIEF2, SMCR7

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Galerie der Validierungsdaten

• 

  WB analysis using 16413-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 16413-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 16413-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 16413-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16413-1-AP (SMCR7/MID49 antibody at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, HepG2-Zellen, HT-1080.Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Skelettmuskelgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 10 publications below
• WB: See 49 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Produktinformation

16413-1-AP bindet in WB, IHC, IF, ELISA SMCR7/MID49 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Affe, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMCR7/MID49 fusion protein Ag9531
• Vollständiger Name: Smith-Magenis syndrome chromosome region, candidate 7
• Berechnetes Molekulargewicht: 454 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC014973
• Gene symbol: SMCR7
• Gene ID (NCBI): 125170
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMCR7, also named as MID49, is a 49 kDa mitochondrial outer membrane protein which regulates mitochondrial morphology. SMCR7 can directly recruit the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface. SMCR7 has two isoforms.

Publikationen

Species	Application	Title
human		Science; Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.; Authors - Shun Nagashima
mouse	WB	Cell Metab; Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.; Authors - Xiuying Zhong
monkey	WB	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KO Validated
human	WB	Autophagy; Loss of MIEF1/MiD51 confers susceptibility to BAX-mediated cell death and PINK1-PRKN-dependent mitophagy.; Authors - Hongxu Xian ; KD Validated
mouse	WB,IF	J Cell Biol; The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission.; Authors - Ruchika Anand
human	WB	EMBO J; FUNDC1 regulates mitochondrial dynamics at the ER-mitochondrial contact site under hypoxic conditions.; Authors - Wenxian Wu ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Chun (Verified Customer) (07-03-2019)

This antibody is very good.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2,000
• Cell Tissue Type: HEK293 and HeLa cells

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