SMARCE1 Polyklonaler Antikörper

SMARCE1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 10814-1-AP

Synonyme

BAF57, BRG1 associated factor 57, SMARCE1

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Galerie der Validierungsdaten

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  WB analysis of Jurkat using 10814-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10814-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10814-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10814-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10814-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10814-1-AP (SMARCE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 10814-1-AP

  IP result of anti-SMARCE1 (IP:10814-1-AP, 4ug; Detection:10814-1-AP 1:800) with HEK-293 cells lysate 880ug.

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  IHC staining of human prostate cancer using 10814-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10814-1-AP (SMARCE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10814-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10814-1-AP (SMARCE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HEK-293-Zellen, HeLa-Zellen, MCF-7-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

10814-1-AP bindet in WB, IP, IHC, ELISA SMARCE1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMARCE1 fusion protein Ag1161
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily e, member 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 50-55 kDa
• GenBank-Zugangsnummer: BC007082
• Gene symbol: SMARCE1
• Gene ID (NCBI): 6605
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Via antagonizing chromatin-mediated transcriptional repression, the SWI/SNF complex in S. cerevisiae and Drosophila is thought to facilitate transcriptional activation of specific genes. The BRG1/brm-associated factors, or SMARCE1, complex in mammals is functionally related to SWI/SNF and consists of 9 to 12 subunits, some of which are homologous to SWI/SNF subunits. It contains a DNA-binding HMG domain and performs multiple biological functions, including DNA-, protein- and chromatin-binding, transcription coactivating activity, chromatin modification, DNA-dependent negative regulation of transcription, regulation of transcription of the RNA polymerase II promoter. In addition, SMARCE1, as an ER (estrogen receptor) subtype-selective modulator, regulates ER mediated transcription in a ligand-dependent manner and also directly binds to AR (androgen receptor) to alter its activity. The calculated molecular weight of SMARCE1 is 47 kDa, but modified SMARCE1 is about 55 kDa.

Publikationen

Species	Application	Title
mouse	WB	iScience; WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation; Authors - Kai Song