SMARCD3 Polyklonaler Antikörper

SMARCD3 Polyklonal Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Kat-Nr. : 12838-1-AP

Synonyme

Rsc6p, CRACD3, BRG1-associated factor 60C, BRG1 associated factor 60C, BAF60C

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Galerie der Validierungsdaten

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  WB analysis using 12838-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 12838-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12838-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12838-1-AP (SMARCD3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 12838-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12838-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12838-1-AP (SMARCD3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human heart using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human heart tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human heart using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human heart tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse pancreas using 12838-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using 12838-1-AP (SMARCD3 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, BxPC-3-Zellen, C6-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Herzgewebe, Maus-Pankreasgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below

Produktinformation

12838-1-AP bindet in WB, IHC, IF-P, ELISA SMARCD3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMARCD3 fusion protein Ag3896
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily d, member 3
• Berechnetes Molekulargewicht: 54 kDa
• Beobachtetes Molekulargewicht: 54-55 kDa
• GenBank-Zugangsnummer: BC002628
• Gene symbol: SMARCD3
• Gene ID (NCBI): 6604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCD3 is a component of the SWI/SNF (mating type switching/sucrose non-fermenting) chromatin remodeling complex, which evolutionarily conserved from yeast to human, and all complexes contain a core set of conserved components, including BRG1/Brm-associated factors (BAFs) and a DNA-dependent SWI2/SNF2-like ATPase, which enables chromatin remodeling. SMARCD3 isoforms bind to several nuclear receptors and transcription factors of various families. SMARCD3 proteins interact in a ligand-independent manner with peroxisome proliferator- activated receptor gamma and enhance its transcriptional activity.

Publikationen

Species	Application	Title
human	WB	Cell Discov; m6Am methyltransferase PCIF1 is essential for aggressiveness of gastric cancer cells by inhibiting TM9SF1 mRNA translation.; Authors - Wei Zhuo
mouse	WB	Cardiovasc Res; The G protein coupled receptor Apj is expressed in the second heart field and regulates Cerberus-Baf60C axis in embryonic stem cell cardiomyogenesis.; Authors - D'Aniello Cristina C
human	WB	Transl Oncol; SNF5, a core subunit of SWI/SNF complex, regulates melanoma cancer cell growth, metastasis, and immune escape in response to matrix stiffness.; Authors - Ying Chen
human	WB	Cells; The SMARCD Family of SWI/SNF Accessory Proteins Is Involved in the Transcriptional Regulation of Androgen Receptor-Driven Genes and Plays a Role in Various Essential Processes of Prostate Cancer; Authors - Iris E Ertl
mouse	WB	Nat Cardiovasc Res; Translational landscape of direct cardiac reprogramming reveals a role of Ybx1 in repressing cardiac fate acquisition; Authors - Yifang Xie