BAF170 Polyklonaler Antikörper

BAF170 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12018-1-AP

Synonyme

SMARCC2, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2, Rsc8, CRACC2, BRG1-associated factor 170

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 12018-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 12018-1-AP (SMARCC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12018-1-AP

  IP result of anti-BAF170 (IP:12018-1-AP, 4ug; Detection:12018-1-AP 1:700) with HeLa cells lysate 3200ug.

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  IHC staining of human medulloblastoma using 12018-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12018-1-AP (SMARCC2 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12018-1-AP

  Immunofluorescent analysis of HepG2 cells, using SMARCC2 antibody 12018-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Medulloblastom-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

12018-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA BAF170 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: BAF170 fusion protein Ag2634
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 2
• Berechnetes Molekulargewicht: 1214 aa, 132 kDa
• Beobachtetes Molekulargewicht: 170 kDa
• GenBank-Zugangsnummer: BC013045
• Gene symbol: BAF170
• Gene ID (NCBI): 6601
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

BAF170, also named as SWI/SNF complex 170 kDa subunit or SMARCC2, is a 1214 amino acid protein, which belongs to the SMARCC family. BAF170 is ubiquitously expressed. BAF170 is involved in transcriptional activation and repression of select genes by chromatin remodeling. BAF170 may be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. BAF170 belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). The calcualted molecular weight of BAF170, is 133 kDa, but modified BAF170 is about 170 kDa. (PMID: 8804307)

Publikationen

Species	Application	Title
		Mol Cell; SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.; Authors - Shibin Hu
human	WB,IP	Aging (Albany NY); FBXO28 promotes proliferation, invasion, and metastasis of pancreatic cancer cells through regulation of SMARCC2 ubiquitination; Authors - Songbai Liu
human	WB	Biochem Biophys Res Commun; SMARCC2 silencing suppresses oncogenic activation through modulation of chromatin accessibility in breast cancer; Authors - Zhaoran Sun ; KD Validated