SMARCAL1 Polyklonaler Antikörper

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• KD/KO Validated

SMARCAL1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 12513-1-AP

Synonyme

HARP, HepA related protein, HHARP, SMARCAL1

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Galerie der Validierungsdaten

• 

  WB analysis of HEK-293 using 12513-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12513-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12513-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12513-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12513-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12513-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12513-1-AP (SMARCAL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen, Maushodengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Produktinformation

12513-1-AP bindet in WB, IP, ELISA SMARCAL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMARCAL1 fusion protein Ag3194
• Vollständiger Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a-like 1
• Berechnetes Molekulargewicht: 954 aa, 106 kDa
• Beobachtetes Molekulargewicht: 106 kDa
• GenBank-Zugangsnummer: BC016482
• Gene symbol: SMARCAL1
• Gene ID (NCBI): 50485
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMARCAL1 is identified as a factor that favors tumor immune evasion by a dual mechanism involving both the suppression of innate immune signaling and the induction of PD-L1-mediated immune checkpoint responses. Mechanistically, SMARCAL1 limits endogenous DNA damage, thereby suppressing cGAS-STING-dependent signaling during cancer cell growth. Simultaneously, it cooperates with the AP-1 family member JUN to maintain chromatin accessibility at a PD-L1 transcriptional regulatory element, thereby promoting PD-L1 expression in cancer cells (PMID: 38301646).

Publikationen

Species	Application	Title
human	WB,IP	Sci Total Environ; LncRNA MT1DP promotes cadmium-induced DNA replication stress by inhibiting chromatin recruitment of SMARCAL1.; Authors - Wenya Feng
human	WB	J Exp Clin Cancer Res; RETSAT associates with DDX39B to promote fork restarting and resistance to gemcitabine based chemotherapy in pancreatic ductal adenocarcinoma; Authors - Qiu Tu ; KD Validated