SMAD4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SMAD4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (5)

Anwendung

WB, IHC, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

89

Kat-Nr. : 10231-1-AP

Synonyme

DPC4, hSMAD4, JIP, MAD homolog 4, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

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Galerie der Validierungsdaten

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  WB analysis using 10231-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10231-1-AP

  WB result of SMAD4 antibody (10231-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD4 transfected HEK-293 cells.

• 

  WB analysis of NIH/3T3 using 10231-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10231-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10231-1-AP (SMAD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10231-1-AP

  IP result of anti-SMAD4 (IP:10231-1-AP, 4ug; Detection:10231-1-AP 1:1500) with HeLa cells lysate 1320 ug.

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  IHC staining of human testis using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10231-1-AP (SMAD4 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human placenta using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10231-1-AP (SMAD4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human tonsillitis using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10231-1-AP (SMAD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10231-1-AP (SMAD4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10231-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10231-1-AP (SMAD4 Antibody) at dilution of 1:50 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: COLO 320-Zellen, HCT 116-Zellen, HEK-293T-Zellen, HEK-293-Zellen, Mauslebergewebe, Neuro-2a-Zellen, NIH/3T3-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Hodengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 77 publications below
• IHC: See 10 publications below
• IF: See 6 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below
• ChIP: See 3 publications below

Produktinformation

10231-1-AP bindet in WB, IHC, IF, IP, CoIP, ChIP, ELISA SMAD4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMAD4 fusion protein Ag0299
• Vollständiger Name: SMAD family member 4
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC002379
• Gene symbol: SMAD4
• Gene ID (NCBI): 4089
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against an internal region of human SMAD4.

Publikationen

Species	Application	Title
human	WB	Nat Commun; KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics; Authors - Mai Abdel Mouti
human	WB,IP,IF	Transl Res; Deubiquitinase USP13 promotes extracellular matrix expression by stabilizing Smad4 in lung fibroblast cells.; Authors - Xinxin Liao ; KD Validated
human	WB	Theranostics; PMID: 28839463; Authors - Junlong Zhuang
rat	WB,IF	Acta Pharmacol Sin; Role of thrombospondin-1 in high-salt-induced mesenteric artery endothelial impairment in rats; Authors - Fang-Fang Xu ; KD Validated
human	WB	EMBO Rep; Mouse endothelial OTUD1 promotes angiotensin II-induced vascular remodeling by deubiquitinating SMAD3; Authors - Zhuqi Huang
human	WB	Stem Cell Reports; Gene Correction Reverses Ciliopathy and Photoreceptor Loss in iPSC-Derived Retinal Organoids from Retinitis Pigmentosa Patients.; Authors - Wen-Li Deng