SMAD2 Monoklonaler Antikörper

SMAD2 Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

7

CloneNo.

2H10C7

Kat-Nr. : 67343-1-Ig

Synonyme

hMAD 2, hSMAD2, JV18, JV18 1, MAD homolog 2, Mad related protein 2, MADH2, MADR2, Mothers against DPP homolog 2, SMAD 2, SMAD family member 2, SMAD2

---

Galerie der Validierungsdaten

• 

  WB analysis using 67343-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67343-1-Ig (SMAD2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis using 67343-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67343-1-Ig (SMAD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 67343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67343-1-Ig (SMAD2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 67343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67343-1-Ig (SMAD2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 67343-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67343-1-Ig (SMAD2 antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HEK-293-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, LNCaP-Zellen, NIH/3T3-Zellen, PC-12-Zellen, RAW 264.7-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

67343-1-Ig bindet in WB, IHC, IF, ELISA SMAD2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SMAD2 fusion protein Ag19542
• Vollständiger Name: SMAD family member 2
• Berechnetes Molekulargewicht: 467 aa, 52 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC014840
• Gene symbol: SMAD2
• Gene ID (NCBI): 4087
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SMAD2, also named as MADH2 and MADR2, belongs to the dwarfin/SMAD family, contains 1 MH1 (MAD homology 1) domain and 1 MH2 (MAD homology 2) domain. SMAD2 is a receptor-regulated SMAD(R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. This protein may act as a tumor suppressor in colorectal carcinoma. It is phosphorylated on one or several of Thr-220, Ser-245, Ser-250, and Ser-255. In response to TGF-beta, It is phosphorylated on Ser-465/467 by TGF-beta and activin type 1 receptor kinases, and then able to interact with SMURF2, recruiting other proteins, such as SNON, for degradation. In response to decorin, the naturally occurring inhibitor of TGF-beta signaling, it is phosphorylated on Ser-240 by CaMK2. It is phosphorylated by MAPK3 upon EGF stimulation; which increases transcriptional activity and stability, and is blocked by calmodulin. In response to TGF-beta, it is ubiquitinated by NEDD4L, which promotes its degradation. In response to TGF-beta signaling, it is acetylated on Lys-19 by coactivators, which increases transcriptional activity. The molecular weight of unphosphorylated forms of Smad2 is 52 kDa and phosphorylated forms of Smad2 is 58 kDa. (PMID: 9006934)

Publikationen

Species	Application	Title
rat	WB	J Cell Mol Med; Mesenchymal stem cells ameliorate silica-induced pulmonary fibrosis by inhibition of inflammation and epithelial-mesenchymal transition.; Authors - Jingjing Wei
rat	WB	Pediatr Pulmonol; Effect of electro-acupuncture at ST 36 on maternal food restriction-induced lung phenotype in rat offspring.; Authors - Qiujie Mou
rat	WB	RSC Adv; Quercetin ameliorates podocyte injury via inhibition of oxidative stress and the TGF-β1/Smad pathway in DN rats; Authors - Fanfan Gao
mouse	WB	Evid Based Complement Alternat Med; The Traditional Chinese Medicine Formula FTZ Protects against Cardiac Fibrosis by Suppressing the TGFβ1-Smad2/3 Pathway.; Authors - Yue Zhang
rat	WB	Phytomedicine; The application of proteomics and metabolomics to reveal the molecular mechanism of Nutmeg-5 in ameliorating cardiac fibrosis following myocardial infarction; Authors - Tingting Yan
human	WB	Arthritis Res Ther; SMAD2 inhibits pyroptosis of fibroblast-like synoviocytes and secretion of inflammatory factors via the TGF-β pathway in rheumatoid arthritis; Authors - Xingxing Mao