SMAD4 Polyklonaler Antikörper

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• KD/KO Validated

SMAD4 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 51144-1-AP

Synonyme

DPC4, hSMAD4, JIP, MADH4, Mothers against DPP homolog 4, SMAD 4, SMAD family member 4, SMAD4

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 51144-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 51144-1-AP (SMAD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 cells using 51144-1-AP

  WB results of SMAD4 antibody (51144-1-AP, 1:1000) with si-Control and si-SMAD4 transfected HepG2 cells.

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  WB analysis of PC-3 using 51144-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 51144-1-AP (SMAD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 51144-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 51144-1-AP (SMAD4 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

51144-1-AP bindet in WB, IHC, ELISA SMAD4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: SMAD family member 4
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC002379
• Gene symbol: SMAD4
• Gene ID (NCBI): 4089
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Mammalian homologs of the Drosophila Mad gene include Smad1, Smad2, Smad3, Smad4 (DPC4), Smad5, Smad6, Smad7 and Smad8. Smad1 and Smad5 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interactionwith various Smad family members . Smad6 and Smad7 regulate the response to activin/ TGF signaling by interfering with TGF -mediated phosphorylation of other Smad family members. This antibody is a rabbit polyclonal antibody raised against a peptide (25-43aa) mapping within human SMAD4. It is specific to SMAD4.

Publikationen

Species	Application	Title
rat	WB	Exp Ther Med; Naringin attenuates renal interstitial fibrosis by regulating the TGF-β/Smad signaling pathway and inflammation.; Authors - Ruichen Wang
rat	WB	Open Life Sci; NR4A1 inhibits the epithelial-mesenchymal transition of hepatic stellate cells: Involvement of TGF-β-Smad2/3/4-ZEB signaling.; Authors - Qian Huang
rat	WB	Exp Ther Med; MicroRNA-122 inhibits epithelial-mesenchymal transition of hepatic stellate cells induced by the TGF-β1/Smad signaling pathway.; Authors - Bianqiao Cheng