SMAD1 Polyklonaler Antikörper

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• KD/KO Validated

SMAD1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

27

Kat-Nr. : 10429-1-AP

Synonyme

MADH1, JV4-1, JV41, hSMAD1, BSP-1

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Galerie der Validierungsdaten

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  WB analysis using 10429-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10429-1-AP

  WB result of SMAD1 antibody (10429-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMAD1 transfected HEK-293 cells.

• 

  WB analysis of HepG2 using 10429-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10429-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10429-1-AP (SMAD1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10429-1-AP

  IP result of anti-SMAD1 (IP:10429-1-AP, 4ug; Detection:10429-1-AP 1:500) with mouse heart tissue lysate 8000ug.

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  IHC staining of human colon using 10429-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 10429-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 10429-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 10429-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10429-1-AP (SMAD1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 10429-1-AP

  Immunofluorescent analysis of HeLa cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 10429-1-AP

  Immunofluorescent analysis of HepG2 cells using 10429-1-AP (SMAD1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HT-1080.Zellen, HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Mausherzgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Kolongewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 27 publications below

Produktinformation

10429-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SMAD1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMAD1 fusion protein Ag0701
• Vollständiger Name: SMAD family member 1
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC001878
• Gene symbol: SMAD1
• Gene ID (NCBI): 4086
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS). Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase

Publikationen

Species	Application	Title
mouse	WB	Carbohydr Polym; In vitro and in silico anti-osteoporosis activities and underlying mechanisms of a fructan, ABW90-1, from Achyranthes bidentate.; Authors - Tianyu Li
mouse	WB	Cell Death Discov; TAZ promotes osteogenic differentiation of mesenchymal stem cells line C3H10T1/2, murine multi-lineage cells lines C2C12, and MEFs induced by BMP9; Authors - Huakun Huang
mouse	WB	Front Immunol; Silencing SOCS3 Markedly Deteriorates Spondyloarthritis in Mice Induced by Minicircle DNA Expressing IL23.; Authors - Yuhai Chen
mouse	WB	Front Mol Neurosci; Tcf4 Controls Neuronal Migration of the Cerebral Cortex through Regulation of Bmp7.; Authors - Tianda Chen
human	WB	IUBMB Life; LncRNA RP11-301G19.1 is required for the maintenance of vascular smooth muscle cell contractile phenotype via sponging miR-17-5P/ATOH8 axis; Authors - Shuning Hao
mouse	WB	Ecotoxicol Environ Saf; Exosomal miR-125a-5p derived from silica-exposed macrophages induces fibroblast transdifferentiation.; Authors - Di Wang