SLC9A9 Polyklonaler Antikörper

SLC9A9 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 13718-1-AP

Synonyme

NHE-9, NHE9, NHE 9, Nbla00118, Na(+)/H(+) exchanger 9

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Galerie der Validierungsdaten

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  WB analysis of Raji using 13718-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 13718-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13718-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13718-1-AP (SLC9A9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 13718-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13718-1-AP (SLC9A9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 13718-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 13718-1-AP (SLC9A9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:600
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

13718-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA SLC9A9 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC9A9 fusion protein Ag4660
• Vollständiger Name: solute carrier family 9 (sodium/hydrogen exchanger), member 9
• Berechnetes Molekulargewicht: 645 aa, 73 kDa
• Beobachtetes Molekulargewicht: 66-73 kDa
• GenBank-Zugangsnummer: BC035779
• Gene symbol: SLC9A9
• Gene ID (NCBI): 285195
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Cell Commun Signal; MicroRNA-135a regulates NHE9 to inhibit proliferation and migration of glioblastoma cells.; Authors - Daniela M Gomez Zubieta
human	WB	Am J Med Genet B Neuropsychiatr Genet; Autism spectrum disorder traits in Slc9a9 knock-out mice.; Authors - Lina Yang
human	WB, CoIP	Atten Defic Hyperact Disord; Effect of disease-associated SLC9A9 mutations on protein-protein interaction networks: implications for molecular mechanisms for ADHD and autism.; Authors - Yanli Zhang-James