SLC4A4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC4A4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 11885-1-AP

Synonyme

Na(+)/HCO3(-) cotransporter, Na(+)/HCO3( ) cotransporter, kNBC1, KNBC, HNBC1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 11885-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 11885-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11885-1-AP (SLC4A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11885-1-AP

  IP result of anti-SLC4A4 (IP:11885-1-AP, 4ug; Detection:11885-1-AP 1:400) with HEK-293 cells lysate 1200ug.

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  IHC staining of human kidney using 11885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 11885-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 11885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse small intestine using 11885-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11885-1-AP (SLC4A4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11885-1-AP

  Immunofluorescent analysis of HepG2 cells, using SLC4A4 antibody 11885-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mausnierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, Maus-Dünndarmgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 16 publications below
• IHC: See 8 publications below
• IF: See 1 publications below

Produktinformation

11885-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SLC4A4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC4A4 fusion protein Ag2490
• Vollständiger Name: solute carrier family 4, sodium bicarbonate cotransporter, member 4
• Berechnetes Molekulargewicht: 121 kDa
• Beobachtetes Molekulargewicht: 121 kDa
• GenBank-Zugangsnummer: BC030977
• Gene symbol: SLC4A4
• Gene ID (NCBI): 8671
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cell; The mTORC1-SLC4A7 axis stimulates bicarbonate import to enhance de novo nucleotide synthesis.; Authors - Eunus S Ali
mouse	WB	Int J Mol Sci; Modulation of Tubular pH by Acetazolamide in a Ca2+ Transport Deficient Mice Facilitates Calcium Nephrolithiasis.; Authors - Eugenia Awuah Boadi
mouse	IHC	Am J Physiol Gastrointest Liver Physiol; Expression of the regulated isoform of the electrogenic Na+/HCO3- cotransporter, NBCe1, is enriched in pacemaker interstitial cells of Cajal.; Authors - Maria-Gabriela Colmenares Aguilar ; KO Validated
human	IHC	Am J Physiol Renal Physiol; Expression of sodium-dependent dicarboxylate transporter 1 (NaDC1/SLC13A2) in normal and neoplastic human kidney.; Authors - Hyun-Wook Lee
mouse	WB	Front Physiol; Selective Deletion of the Mechanistic Target of Rapamycin From the Renal Collecting Duct Principal Cell in Mice Down-Regulates the Epithelial Sodium Channel.; Authors - Bruce Chen
	WB,IHC	Front Physiol; Expression Regulation of Water Reabsorption Genes and Transcription Factors in the Kidneys of Lepus yarkandensis.; Authors - Shengjie Luo