FATP4 Polyklonaler Antikörper

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• KD/KO Validated

FATP4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 11013-1-AP

Synonyme

ACSVL4, FATP 4, FATP4, Fatty acid transport protein 4, SLC27A4

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 11013-1-AP

  WB result of FATP4 antibody (11013-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-FATP4 transfected HepG2 cells.

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  WB analysis of human colon using 11013-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11013-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 11013-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 11013-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11013-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11013-1-AP (FATP4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 11013-1-AP

  IP result of anti-FATP4 (IP:11013-1-AP, 4ug; Detection:11013-1-AP 1:500) with HepG2 cells lysate 1600ug.

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11013-1-AP (FATP4 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11013-1-AP (FATP4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 11013-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11013-1-AP (FATP4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, humanes Herzgewebe, humanes Kolongewebe, Maus-Kolongewebe, Mauslebergewebe, Ratten-Kolongewebe, Rattenlebergewebe, SMMC-7721-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11013-1-AP bindet in WB, IP, IF, IHC, ELISA FATP4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: FATP4 fusion protein Ag1490
• Vollständiger Name: solute carrier family 27 (fatty acid transporter), member 4
• Berechnetes Molekulargewicht: 72 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC009959
• Gene symbol: FATP4/SLC27A4
• Gene ID (NCBI): 10999
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
rat	WB	Sci Rep; PMID: 28304381; Authors - Wenjing Li
human	WB	Front Immunol; Cathelicidin-WA Facilitated Intestinal Fatty Acid Absorption Through Enhancing PPAR-γ Dependent Barrier Function.; Authors - Xin Zong
mouse	WB	iScience; Celastrol inhibits intestinal lipid absorption by reprofiling the gut microbiota to attenuate high-fat diet-induced obesity.; Authors - Hu Hua
	WB	Int J Mol Sci; Nano-Zn Increased Zn Accumulation and Triglyceride Content by Up-Regulating Lipogenesis in Freshwater Teleost, Yellow Catfish Pelteobagrus fulvidraco.; Authors - Shi-Cheng Ling
mouse	IHC	Biochim Biophys Acta; Uninephrectomy augments the effects of high fat diet induced obesity on gene expression in mouse kidney.; Authors - Zhibo Gai
mouse,pig	WB	Cells; LPS Inhibits Fatty Acid Absorption in Enterocytes through TNF-α Secreted by Macrophages.; Authors - Heyuan Liu