SLC25A3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC25A3 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte und mehr (1)

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 10420-1-AP

Synonyme

OK/SW cl.48, PHC, Phosphate transport protein, PiC, PTP, SLC25A3

---

Galerie der Validierungsdaten

• 

  WB analysis using 10420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10420-1-AP (SLC25A3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10420-1-AP

  WB result of SLC25A3 antibody (10420-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLC25A3 transfected HeLa cells.

• 

  WB analysis using 10420-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10420-1-AP (SLC25A3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 10420-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10420-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10420-1-AP (SLC25A3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, MOLT-4-Zellen, PC-12-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Produktinformation

10420-1-AP bindet in WB, IHC, ELISA SLC25A3 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A3 fusion protein Ag0667
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier; phosphate carrier), member 3
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 40-43 kDa
• GenBank-Zugangsnummer: BC003504
• Gene symbol: SLC25A3
• Gene ID (NCBI): 5250
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The mitochondrial phosphate carrier SLC25A3 belongs to the SLC25 family , which is the largest family of solute carriers. SLC25A3 encodes the phosphate carrier PiC that catalyses the import of phosphate into mitochondria where it is needed for ATP synthesis. SLC25A3 has two isoforms SLC25A3-A (a heart-type isoform) and SLC25A3-B (a liver-type isoform) as a result of alternative splicing of exon 3. It has been reported that SLC25A3 mutations are associated with mitochondrial phosphate carrier deficiency, respiratory distress and hypertrophic cardiomyopathy. (PMID: 15984930, 27780865, 20877624)

Publikationen

Species	Application	Title
human	IHC	Heliyon; Prognostic value and immunological function of cuproptosis-related genes in lung adenocarcinoma; Authors - Liming Zhang
human,mouse	WB	Exp Gerontol; METTL14-mediated methylation of SLC25A3 mitigates mitochondrial damage in osteoblasts, leading to the improvement of osteoporosis; Authors - Ping Wang ; KD Validated
mouse	WB	Dev Cell; Slc25a3-dependent copper transport controls flickering-induced Opa1 processing for mitochondrial safeguard; Authors - Daisuke Murata