SLC25A12 Polyklonaler Antikörper

SLC25A12 Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26804-1-AP

Synonyme

AGC1, ARALAR, ARALAR1, SLC25A12

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Galerie der Validierungsdaten

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  WB analysis of mouse skeletal muscle using 26804-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26804-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 26804-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 26804-1-AP (SLC25A12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 26804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 26804-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 26804-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 26804-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26804-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 26804-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 26804-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26804-1-AP (SLC25A12 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse heart using 26804-1-AP

  Immunofluorescent analysis of (4% PFA) fixed tissue using 26804-1-AP (SLC25A12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mausnierengewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Magenkrebsgewebe, humanes Pankreaskarzinomgewebe, Mausnierengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26804-1-AP bindet in WB, IHC, IF, ELISA SLC25A12 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC25A12 fusion protein Ag25150
• Vollständiger Name: solute carrier family 25 (mitochondrial carrier, Aralar), member 12
• Berechnetes Molekulargewicht: 75 kDa, 63 kDa
• Beobachtetes Molekulargewicht: 63 kDa
• GenBank-Zugangsnummer: BC016932
• Gene symbol: SLC25A12
• Gene ID (NCBI): 8604
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.