SLC22A18 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SLC22A18 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 11646-2-AP

Synonyme

BWR1A, BWSCR1A, HET, IMPT1, ITM, ORCTL 2, ORCTL2, p45 BWR1A, SLC22A18, SLC22A1L, TSSC5

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 11646-2-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11646-2-AP (SLC22A18 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 11646-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11646-2-AP (SLC22A18 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 11646-2-AP

  IP result of anti-SLC22A18 (IP:11646-2-AP, 4ug; Detection:11646-2-AP 1:600) with mouse brain tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, COLO 320-Zellen
• Erfolgreiche IP: Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Produktinformation

11646-2-AP bindet in WB, IP, IHC, ELISA SLC22A18 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC22A18 fusion protein Ag2245
• Vollständiger Name: solute carrier family 22, member 18
• Berechnetes Molekulargewicht: 408 aa, 43 kDa
• Beobachtetes Molekulargewicht: 40-50 kDa
• GenBank-Zugangsnummer: BC015571
• Gene symbol: SLC22A18
• Gene ID (NCBI): 5002
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC22A18 is a member of the family of polyspecific organic-cation transporters that localize at the apical membrane surface of renal proximal tubules. SLC22A18 is expressed at high levels in the kidney, liver, colon and in fetal renal proximal tubules and present at lower levels in heart, brain and lung. Defects in SLC22A18 are associated with breast and lung cancer and are the cause of rhabdomyosarcoma type 1, a malignant tumor derived from striated muscle. More recently, SLC22A18 has been shown to functions as a tumor suppressor in glioma and represents a candidate biomarker for long-term survival in this disease.

Publikationen

Species	Application	Title
human	WB	Int J Biol Macromol; microRNA-137 functions as a tumor suppressor in human non-small cell lung cancer by targeting SLC22A18.; Authors - Bicheng Zhang ; KD Validated
		Hum Mol Genet; Parent-of-origin tumorigenesis is mediated by an essential imprinted modifier in SDHD-linked paragangliomas: SLC22A18 and CDKN1C are candidate tumor modifiers.; Authors - Attje S Hoekstra
human	IHC	Cancer Epidemiol; Low expression of SLC22A18 predicts poor survival outcome in patients with breast cancer after surgery.; Authors - He Hongyu H