SLC20A1 Polyklonaler Antikörper

SLC20A1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (3)

Anwendung

WB, IP, IF, FC, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 12423-1-AP

Synonyme

Glvr 1, GLVR1, Phosphate transporter 1, PiT 1, PIT1, SLC20A1

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12423-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12423-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12423-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12423-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12423-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12423-1-AP (SLC20A1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 12423-1-AP

  IP result of anti-SLC20A1 (IP:12423-1-AP, 4ug; Detection:12423-1-AP 1:500) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human liver using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 12423-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12423-1-AP (SLC20A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, humanes Nierengewebe, Jurkat-Zellen, K-562-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Lymphomgewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• FC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12423-1-AP bindet in WB, IP, IF, FC, IHC, CoIP, ELISA SLC20A1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC20A1 fusion protein Ag3084
• Vollständiger Name: solute carrier family 20 (phosphate transporter), member 1
• Berechnetes Molekulargewicht: 679 aa, 74 kDa
• Beobachtetes Molekulargewicht: 85 kDa
• GenBank-Zugangsnummer: BC019944
• Gene symbol: SLC20A1
• Gene ID (NCBI): 6574
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SLC20A1 (also known as PiT1) is a high-affinity sodium-dependent phosphate (Pi) import protein. It is widely expressed and plays a fundamental housekeeping role in phosphate transport, such as absorbing phosphate from interstitial fluid for normal cellular functions. SLC20A1 is involved in cell proliferation, tumor growth and cell signaling independently of its transport function. SLC20A1 also functions as a retroviral receptor, causing human cells to be susceptible to infection by gibbon ape leukemia virus, simian sarcoma-associated virus, feline leukemia virus subgroup B, and 10A1 murine leukemia virus.

Publikationen

Species	Application	Title
mouse	FC	Nat Cancer; GAP43-dependent mitochondria transfer from astrocytes enhances glioblastoma tumorigenicity; Authors - Dionysios C Watson
human,mouse	WB,CoIP	Theranostics; SGK3 promotes vascular calcification via Pit-1 in chronic kidney disease; Authors - Qing-Qing Dong
human	WB	FASEB J; PMID: 27565711; Authors - Tuyet Thi Nguyen
zebrafish	IF	Front Cell Dev Biol; SLC20A1 Is Involved in Urinary Tract and Urorectal Development.; Authors - Johanna Magdalena Rieke
human	WB	J Biol Chem; Phosphate (Pi)-regulated heterodimerization of the high-affinity sodium-dependent Pi transporters PiT1/Slc20a1 and PiT2/Slc20a2 underlies extracellular Pi sensing independently of Pi uptake.; Authors - Nina Bon
rat	WB	Am J Physiol Heart Circ Physiol; Oxidative Stress by Ca2+ Overload is Critical forPhosphate-Induced Vascular Calcification.; Authors - Nhung Thi Nguyen

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

anwesh (Verified Customer) (07-29-2019)

The protein was found at the right size as a smear. The protein undergoes heavy glycosylation, so that is expected. No additional background bands were observed around the size range.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HEK293T

SLC20A1 Antibody Western Blot, validation (1:500 dilution) in HEK293T (Cat no:12423-1-AP)