SLC1A4 Polyklonaler Antikörper

SLC1A4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13067-2-AP

Synonyme

ASCT 1, ASCT1, SATT, SLC1A4

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Galerie der Validierungsdaten

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  WB analysis using 13067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 13067-2-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13067-2-AP (SLC1A4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 13067-2-AP

  IP result of anti-SLC1A4 (IP:13067-2-AP, 4ug; Detection:13067-2-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 13067-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13067-2-AP (SLC1A4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, humanes Skelettmuskelgewebe, Maushirngewebe, Mausnierengewebe, Rattenhirngewebe, SW480-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Nierengewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

13067-2-AP bindet in WB, IP, IHC, ELISA SLC1A4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC1A4 fusion protein Ag3763
• Vollständiger Name: solute carrier family 1 (glutamate/neutral amino acid transporter), member 4
• Berechnetes Molekulargewicht: 532 aa, 56 kDa
• Beobachtetes Molekulargewicht: 62-70 kDa
• GenBank-Zugangsnummer: BC026216
• Gene symbol: SLC1A4
• Gene ID (NCBI): 6509
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Mol Cancer; CircMYH9 drives colorectal cancer growth by regulating serine metabolism and redox homeostasis in a p53-dependent manner; Authors - Xin Liu
mouse	WB	Ecotoxicol Environ Saf; Toxic effects of maternal cadmium exposure on the metabolism and transport system of amino acids in the maternal livers; Authors - Peng Xu
human	WB	Front Oncol; SLC1A4: A Powerful Prognostic Marker and Promising Therapeutic Target for HCC.; Authors - Xiaozhen Peng
mouse	WB	Res Sq; Generation and characterization of a knock-in mouse model for Spastic Tetraplegia, Thin Corpus Callosum, and Progressive Microcephaly (SPATCCM); Authors - Megan L Ratz