SLC19A3 Polyklonaler Antikörper

SLC19A3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

10

Rezensionen (2)

Kat-Nr. : 13407-1-AP

Synonyme

SLC19A3, Thiamine transporter 2, ThTr 2, THTR2

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Galerie der Validierungsdaten

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  WB analysis using 13407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 13407-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 13407-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 13407-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13407-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 13407-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 13407-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13407-1-AP (SLC19A3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 13407-1-AP

  IP result of anti-SLC19A3 (IP:13407-1-AP, 4ug; Detection:13407-1-AP 1:600) with mouse liver tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe, 3T3-L1-Zellen, humanes Plazenta-Gewebe, Mausnierengewebe, Maus-Skelettmuskelgewebe, Rattenherzgewebe, Rattenlebergewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 10 publications below
• IF: See 1 publications below

Produktinformation

13407-1-AP bindet in WB, IP, IF, ELISA SLC19A3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SLC19A3 fusion protein Ag4053
• Vollständiger Name: solute carrier family 19, member 3
• Berechnetes Molekulargewicht: 496 aa, 56 kDa
• Beobachtetes Molekulargewicht: 63-70 kDa
• GenBank-Zugangsnummer: BC032014
• Gene symbol: SLC19A3
• Gene ID (NCBI): 80704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human,mouse	WB	Sci Transl Med; CPEB alteration and aberrant transcriptome-polyadenylation lead to a treatable SLC19A3 deficiency in Huntington's disease.; Authors - Sara Picó
human	WB	Nat Commun; Enterotoxigenic Escherichia coli heat-labile toxin drives enteropathic changes in small intestinal epithelia; Authors - Alaullah Sheikh
human	WB	Front Cell Dev Biol; Impact of Metformin Treatment on Human Placental Energy Production and Oxidative Stress.; Authors - Jane L Tarry-Adkins
human	WB,IF	J Nutr Biochem; Up-regulation of vitamin B1 homeostasis genes in breast cancer.; Authors - Zastre Jason A JA
human	WB	J Biol Chem; Hypoxia inhibits colonic uptake of the microbiota-generated forms of vitamin B1 via HIF-1α-mediated transcriptional regulation of their transporters.; Authors - Subrata Sabui
mouse	WB	Front Pharmacol; Chronic Voluntary Alcohol Drinking Causes Anxiety-like Behavior, Thiamine Deficiency, and Brain Damage of Female Crossed High Alcohol Preferring Mice.; Authors - Hong Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Valliammai (Verified Customer) (02-15-2023)

Results are highly reliable. Thanks for the service.

• Applications: Western Blot, Immunofluorescence, Protein purification

FH

Selvaraj (Verified Customer) (12-20-2022)

We are frequently using this antibody in our lab

• Applications: Western Blot
• Cell Tissue Type: Human intestinal cell lines