NKCC1,SLC12A2 Polyklonaler Antikörper

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• KD/KO Validated

NKCC1,SLC12A2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

22

Rezensionen (1)

Kat-Nr. : 13884-1-AP

Synonyme

Basolateral Na K Cl symporter, BSC, BSC2, NKCC1, SLC12A2

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Galerie der Validierungsdaten

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  WB analysis using 13884-1-AP

  Mouse brain total extract lysate and 37°C incubated mouse brain membrane extract lysate were subjected to SDS PAGE followed by western blot with 13884-1-AP (NKCC1,SLC12A2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 13884-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13884-1-AP (NKCC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, bei 37°C inkubiertes Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 11 publications below
• IHC: See 9 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Produktinformation

13884-1-AP bindet in WB, IP, IF, IHC, ELISA NKCC1,SLC12A2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: NKCC1,SLC12A2 fusion protein Ag4991
• Vollständiger Name: solute carrier family 12 (sodium/potassium/chloride transporters), member 2
• Berechnetes Molekulargewicht: 131 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC033003
• Gene symbol: NKCC1/SLC12A2
• Gene ID (NCBI): 6558
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The Na-K-Cl cotransporter sodium-potassium-chloride cotransporter 1 (NKCC1), encoded by the SLC12A2 gene, is an intensively studied member of the CCC family. NKCC1 is a membrane protein and plays fundamental roles in regulating trans-epithelial ion movement, cell volume, chloride homeostasis and neuronal excitability. NKCC1 assembles into a dimer, with the first ten transmembrane (TM) helices harboring the transport core and TM11-TM12 helices lining the dimer interface(PMID: 32081947). The observed molecular weight of NKCC1 is about 140 kDa, glycosylated NKCC1 is detected a band at ∼170 kDa (PMID: 22723696).

Publikationen

Species	Application	Title
mouse	WB,IHC	Science; A causative link between inner ear defects and long-term striatal dysfunction.; Authors - Michelle W Antoine
mouse	IHC	PLoS Biol; Early uneven ear input induces long-lasting differences in left-right motor function.; Authors - Michelle W Antoine
mouse	IHC	PLoS Biol; The NKCC1 ion transporter modulates microglial phenotype and inflammatory response to brain injury in a cell-autonomous manner.; Authors - Krisztina Tóth
mouse	WB,IHC	J Neurosci; The severity of vestibular dysfunction in deafness as a determinant of comorbid hyperactivity or anxiety.; Authors - Michelle W Antoine
mouse	IF	Front Mol Neurosci; Unilateral optogenetic kindling of hippocampus leads to more severe impairments of the inhibitory signaling in the contralateral hippocampus; Authors - Fabio Cesar Tescarollo
human	WB	Sci Rep; Comprehensive analyses of solute carrier family members identify SLC12A2 as a novel therapy target for colorectal cancer; Authors - Dan-Yang Chen ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Valentina (Verified Customer) (02-01-2023)

Multiple bands were detectable around 100 - 150 kDa, with different protocols and gels. The antibody is definitely working even if not optimally, probably necessary to try with a higher dilution and a lower amount of protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Choroid plexus homogenates