SLAM/CD150 Monoklonaler Antikörper

SLAM/CD150 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgM

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

1

CloneNo.

5B4G1

Kat-Nr. : 60043-1-Ig

Synonyme

CD150, CDw150, IPO 3, SLAM, SLAMF1

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Galerie der Validierungsdaten

• 

  WB analysis of Raji using 60043-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 60043-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 60043-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60043-1-Ig (SLAM/CD150 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HUVEC-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below

Produktinformation

60043-1-Ig bindet in WB, ELISA SLAM/CD150 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: Affe
• Wirt / Isotyp: Maus / IgM
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SLAM/CD150 fusion protein Ag1386
• Vollständiger Name: signaling lymphocytic activation molecule family member 1
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: BC012602
• Gene symbol: SLAMF1
• Gene ID (NCBI): 6504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Caprylsäure/Ammoniumsulfat-Präzipitation
• Lagerungspuffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Signaling lymphocytic activation molecule (SLAM, also known as SLAMF1 or CD150) is a 70-95 kDa transmembrane glycoprotein that belongs to the immunoglobulin gene superfamily. SLAM is constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes and a proportion of B-cells, and is rapidly induced on naive T-cells after activation. SLAM is involved in T cell stimulation and cytokine production, and may play an important role in the regulation of immune responses to pathogens.

Publikationen

Species	Application	Title
monkey	WB	Animals (Basel); Signaling Lymphocytic Activation Molecule Family Member 1 Inhibits Porcine Reproductive and Respiratory Syndrome Virus Replication; Authors - Haofei Song ; KD Validated