SKP1 Polyklonaler Antikörper

SKP1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Rezensionen (1)

Kat-Nr. : 10990-2-AP

Synonyme

OCP2, OCP II, OCP 2, NET19, EMC19

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Galerie der Validierungsdaten

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  WB analysis using 10990-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10990-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10990-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10990-2-AP (SKP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human gliomas using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 10990-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10990-2-AP (SKP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 10990-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SKP1 antibody (10990-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, humanes Hirngewebe, Maushirngewebe, Maushodengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10990-2-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA SKP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SKP1 fusion protein Ag1457
• Vollständiger Name: S-phase kinase-associated protein 1
• Berechnetes Molekulargewicht: 19 kDa
• Beobachtetes Molekulargewicht: 19 kDa
• GenBank-Zugangsnummer: BC009839
• Gene symbol: SKP1
• Gene ID (NCBI): 6500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SKP1(S-phase kinase-associated protein 1) is also named as EMC19, OCP2, SKP1A, TCEB1L,belongs to the SKP1 family.It is an essential component of the SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription.

Publikationen

Species	Application	Title
human	WB	Elife; Elevated FBXO45 promotes liver tumorigenesis through enhancing IGF2BP1 ubiquitination and subsequent PLK1 upregulation.; Authors - Xiao-Tong Lin
human	WB,IHC	Pharmacol Res; Brusatol has therapeutic efficacy in non-small cell lung cancer by targeting Skp1 to inhibit cancer growth and metastasis.; Authors - Shangping Xing
human	WB	Mol Nutr Food Res; Ethanol Extract of Citrus grandis 'Tomentosa' Exerts Anticancer Effects by Targeting Skp2/p27 Pathway in Non-Small Cell Lung Cancer; Authors - Da Huang
human	WB	J Cell Physiol; The Ubiquitin Ligase SCF(FBXW7α) Promotes ATA3 Degradation.; Authors - Nan Song
human	IHC, WB	J Exp Clin Cancer Res; Long non-coding RNA CCDC183-AS1 acts AS a miR-589-5p sponge to promote the progression of hepatocellular carcinoma through regulating SKP1 expression.; Authors - He Zhu
human	WB	bioRxiv; Recruitment of FBXO22 for Targeted Degradation of NSD2; Authors - David Y Nie

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

WEI (Verified Customer) (05-11-2022)

Weak band but at correct size.

• Applications: Western Blot
• Cell Tissue Type: Mouse Heart