SIRP Beta 1 Polyklonaler Antikörper

SIRP Beta 1 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 11811-1-AP

Synonyme

CD172b, SIRP BETA 1, SIRPB1

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Galerie der Validierungsdaten

• 

  WB analysis using 11811-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11811-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11811-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 11811-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 11811-1-AP (SIRP BETA 1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, A375-Zellen, PC-3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below

Produktinformation

11811-1-AP bindet in WB, IHC, ELISA SIRP Beta 1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SIRP Beta 1 fusion protein Ag2478
• Vollständiger Name: signal-regulatory protein beta 1
• Berechnetes Molekulargewicht: 43 kDa
• Beobachtetes Molekulargewicht: 43 kDa
• GenBank-Zugangsnummer: BC025286
• Gene symbol: SIRPB1
• Gene ID (NCBI): 10326
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SIRP Beta 1 is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP Beta 1 interacts with TYROBP/DAP12, a protein bearing immunoreceptor tyrosine-based activation motifs. SIRP Beta 1 also participates in the recruitment of tyrosine kinase SYK.

Publikationen

Species	Application	Title
human	IHC	PLoS One; SNPs array karyotyping reveals a novel recurrent 20p13 amplification in primary myelofibrosis.; Authors - Visani Giuseppe G
human	IHC	Neuropathology; Immunohistochemical characterization of microglia in Nasu-Hakola disease brains.; Authors - Satoh Jun-Ichi J