SHCBP1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SHCBP1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

13

Kat-Nr. : 12672-1-AP

Synonyme

PAL, SHCBP1

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Galerie der Validierungsdaten

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  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 12672-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12672-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12672-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 12672-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 12672-1-AP (SHCBP1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 12672-1-AP

  IP result of anti-SHCBP1 (IP:12672-1-AP, 4ug; Detection:12672-1-AP 1:500) with Jurkat cells lysate 2400ug.

• 

  IHC staining of human liver cancer using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12672-1-AP (SHCBP1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12672-1-AP (SHCBP1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human tonsillitis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12672-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12672-1-AP (SHCBP1 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, Maus-Thymusgewebe, Neuro-2a-Zellen, Sp2/0-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe, humanes Hodengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 11 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below

Produktinformation

12672-1-AP bindet in WB, IP, IF, IHC, ELISA SHCBP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Hund, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SHCBP1 fusion protein Ag3355
• Vollständiger Name: SHC SH2-domain binding protein 1
• Berechnetes Molekulargewicht: 672 aa, 76 kDa
• Beobachtetes Molekulargewicht: 75-85 kDa
• GenBank-Zugangsnummer: BC030699
• Gene symbol: SHCBP1
• Gene ID (NCBI): 79801
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SHCBP1 was initially identified as a potential gene associated with cell proliferation in fibroblasts. SHCBP1 exerts a crucial physiological function in normal tissue development. Chen et al. showed that SHCBP1 is an essential component of FGF signaling in neural progenitor cells. SHCBP1 is also upregulated during T cell proliferation and regulates CD4+ T cell effector function in vivo. Recently, SHCBP1 was shown to be closely associated with cancer development.

Publikationen

Species	Application	Title
mouse	WB	Nat Neurosci; Impaired neurogenesis alters brain biomechanics in a neuroprogenitor-based genetic subtype of congenital hydrocephalus.; Authors - Phan Q Duy
human	WB,IF,IP	Genomics; SHCBP1 interacting with EOGT enhances O-GlcNAcylation of NOTCH1 and promotes the development of pancreatic cancer.; Authors - Can Yang ; KD Validated
human	WB,IHC	Am J Cancer Res; SHCBP1 regulates STAT3/c-Myc signaling activation to promote tumor progression in penile cancer.; Authors - Miao Mo ; KD Validated
human	WB	J Virol; Measles virus non-structural C protein modulates viral RNA polymerase activity by interacting with a host protein SHCBP1.; Authors - Ito Minako M ; KD Validated
mouse	WB	Mol Carcinog; Knockout of Shcbp1 sensitizes immunotherapy by regulating α-SMA positive cancer-associated fibroblasts; Authors - Qianlin Gu ; KO Validated
human	IF,IHC, WB	J Exp Clin Cancer Res; SHCBP1 promotes synovial sarcoma cell metastasis via targeting TGF-β1/Smad signaling pathway and is associated with poor prognosis.; Authors - Changliang Peng ; KD Validated