SHC Polyklonaler Antikörper

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• KD/KO Validated

SHC Polyklonal Antikörper für WB, IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

11

Kat-Nr. : 10054-1-AP

Synonyme

SH2 domain protein C1, SHC, SHC transforming protein 1, SHC transforming protein 3, SHC transforming protein A, SHC1, SHCA

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Galerie der Validierungsdaten

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  WB analysis using 10054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10054-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 10054-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skin using 10054-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 10054-1-AP (SHC antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 10054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10054-1-AP (SHC antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon cancer using 10054-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10054-1-AP (SHC antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10054-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 10054-1-AP (SHC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C6-Zellen, A431-Zellen, HeLa-Zellen, HUVEC-Zellen, Maushautgewebe, U2OS-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Produktinformation

10054-1-AP bindet in WB, IHC, IF/ICC, ELISA SHC und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SHC fusion protein Ag0103
• Vollständiger Name: SHC (Src homology 2 domain containing) transforming protein 1
• Berechnetes Molekulargewicht: 63 kDa
• Beobachtetes Molekulargewicht: 66 kDa
• GenBank-Zugangsnummer: NM_183001
• Gene symbol: SHC1
• Gene ID (NCBI): 6464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The proto-oncogene SHC family consists of SHCA, SHCB, SHCC, and SHCD4 gene members, which can encode 7 kinds of cohesive molecules. SHC family is widely expressed and functional. The SHC1 gene is located in zone 1, region 2 of human chromosome 1. SHC1 is believed to be involved in inhibiting neuronal apoptosis and regulating the receptor tyrosine kinase pathway. SHC1 sensitizes cancer cells to the 8-Cl-cAMP treatment.

Publikationen

Species	Application	Title
human	WB	Clin Transl Med; Aptamer-SH2 superbinder-based targeted therapy for pancreatic ductal adenocarcinoma.; Authors - An-Dong Liu
mouse	IF	Oxid Med Cell Longev; Protocatechuic Acid-Mediated miR-219a-5p Activation Inhibits the p66shc Oxidant Pathway to Alleviate Alcoholic Liver Injury.; Authors - Rong Fu ; KD Validated
mouse	WB	Aging (Albany NY); RNA-seq analysis of the key long noncoding RNAs and mRNAs related to cognitive impairment after cardiac arrest and cardiopulmonary resuscitation.; Authors - Chan Chen
mouse	WB	Aging (Albany NY); Targeting of miR-96-5p by catalpol ameliorates oxidative stress and hepatic steatosis in LDLr-/- mice via p66shc/cytochrome C cascade.; Authors - Yukun Zhang
rat	WB	J Ethnopharmacol; Dahuang Zhechong pill attenuates hepatic sinusoidal capillarization in liver cirrhosis and hepatocellular carcinoma rat model via MK/integrin signaling pathway; Authors - Chuankui Fu
mouse	WB	Stem Cell Res Ther; Oncostatin M receptor regulates osteoblast differentiation via extracellular signal-regulated kinase/autophagy signaling.; Authors - Jie Zhou