SH3BP5 Monoklonaler Antikörper

SH3BP5 Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, Huhn, human, Kaninchen, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1B9D4

Kat-Nr. : 67841-1-Ig

Synonyme

SAB, SH3 domain binding protein 5, SH3BP 5, SH3BP5

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Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 67841-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of chicken brain using 67841-1-Ig

  chicken brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67841-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 67841-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 67841-1-Ig (SH3BP5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, HeLa-Zellen, Hühnerhirngewebe, Jurkat-Zellen, Kaninchenhirngewebe, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67841-1-Ig bindet in WB, ELISA SH3BP5 und zeigt Reaktivität mit Hausschwein, Huhn, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, Huhn, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SH3BP5 fusion protein Ag30252
• Vollständiger Name: SH3-domain binding protein 5 (BTK-associated)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: BC010123
• Gene symbol: SH3BP5
• Gene ID (NCBI): 9467
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.