SH3BP5 Polyklonaler Antikörper

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• KD/KO Validated

SH3BP5 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 11127-2-AP

Synonyme

SAB, SH3 domain binding protein 5, SH3BP 5, SH3BP5

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Galerie der Validierungsdaten

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  WB analysis of Raji using 11127-2-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 11127-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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  IHC staining of human ovary tumor using 11127-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 11127-2-AP (SH3BP5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Sodium Citrate buffer (pH 6.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:500
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 4 publications below
• WB: See 6 publications below

Produktinformation

11127-2-AP bindet in WB, IHC, ELISA SH3BP5 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH3BP5 fusion protein Ag1592
• Vollständiger Name: SH3-domain binding protein 5 (BTK-associated)
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 32 kDa, 50-83 kDa
• GenBank-Zugangsnummer: BC010123
• Gene symbol: SH3BP5
• Gene ID (NCBI): 9467
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH3BP5(SH3 domain-binding protein 5) is also named as SAB. It is previously isolated in a screen for proteins capable of binding to the Src homology 3 (SH3) domain of Bruton's tyrosine kinase (Btk) and could play a role in targeting JNK and Btk to mitochondria and/or mediating cross-talk between the JNK and Btk signalling pathways. It has 2 isoforms produced by alternative splicing with the molecular mass of 50 kDa and 32 kDa. There are two bands of 83 kDa and 70 kDa can be detected in A431 and the larger species may correspond to splice variants of SH3BP5 or SH3BP5 proteins that have undergone post-translational modification(PMID:12167088). In addition, we detect the band of 32 kDa as the isoform 2 of SH3BP5 and another band of 62 kDa besides the 47 kDa and 80 kDa.

Publikationen

Species	Application	Title
mouse	WB	Hepatology; Liver-specific deficiency of unc-51 like kinase 1 and 2 protects mice from acetaminophen-induced liver injury.; Authors - Yu Sun
mouse	WB	J Hepatol; Sab (Sh3bp5) dependence of JNK mediated inhibition of mitochondrial respiration in palmitic acid induced hepatocyte lipotoxicity.; Authors - Sanda Win ; KD Validated
mouse	WB	Cell Death Dis; JNK interaction with Sab mediates ER stress induced inhibition of mitochondrial respiration and cell death.; Authors - S Win ; KD Validated
human,mouse	WB	Front Pharmacol; Scoparone Improves Nonalcoholic Steatohepatitis Through Alleviating JNK/Sab Signaling Pathway-Mediated Mitochondrial Dysfunction.; Authors - Yuwei Jiang ; KD Validated
human	WB	Biomolecules; Elevated SH3BP5 Correlates with Poor Outcome and Contributes to the Growth of Acute Myeloid Leukemia Cells.; Authors - Minjing Li ; KD Validated
mouse	WB	Biomed Pharmacother; Fluorofenidone protects against acute liver failure in mice by regulating MKK4/JNK pathway; Authors - Lei Gu