SH2D3C Polyklonaler Antikörper

SH2D3C Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12882-1-AP

Synonyme

CHAT, Novel SH2 containing protein 3, NSP3, PRO34088, SH2 domain containing 3C, SH2D3C

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 12882-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 12882-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of human placenta using 12882-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12882-1-AP (SH2D3C antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 12882-1-AP

  IP result of anti-SH2D3C (IP:12882-1-AP, 3ug; Detection:12882-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IP experiment using 12882-1-AP

  IP result of SH2D3C antibody (12882-1-AP for IP and Detection) with mouse brain tissue lysate.

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  IHC staining of human breast cancer using 12882-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12882-1-AP (SH2D3C antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, humanes Hirngewebe, humanes Plazenta-Gewebe
• Erfolgreiche IP: Maushirngewebe,
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IP: See 1 publications below

Produktinformation

12882-1-AP bindet in WB, IP, IHC, ELISA SH2D3C und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SH2D3C fusion protein Ag3887
• Vollständiger Name: SH2 domain containing 3C
• Berechnetes Molekulargewicht: 94 kDa, 86 kDa, 77 kDa
• Beobachtetes Molekulargewicht: 77 kDa
• GenBank-Zugangsnummer: BC027962
• Gene symbol: SH2D3C
• Gene ID (NCBI): 10044
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SH2 domain-containing protein 3C (SH2D3C, or NSP3) gene encodes an adaptor protein and member of a cytoplasmic protein family involved in cell migration. The encoded protein was identified as an Eph receptor-binding protein which may be a positive regulator of TCR signaling (PMID: 10542222). SH2D3C is an adaptor protein that has been implicated in T cell activation and shown to associate with different components of the integrin signaling pathway ex vivo, moreover, its primary in vivo role of Sh2d3c in regulating B cell development and function has also been demonstrated (PMID: 20505138). SH2D3C has 6 isoforms with MW 94, 86, 77 and 55 kDa (refer to UniProt).

Publikationen

Species	Application	Title
rat	WB,IP	Neuroscience; Up-regulation of NSP3 by oligomeric Aβ accelerates neuronal death through Cas-independent Rap1A activation.; Authors - Fujiya Gomi