SGK3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SGK3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 12699-1-AP

Synonyme

SGKL, SGK 3, Serum/glucocorticoid regulated kinase-like isoform 1 variant, CISK

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 12699-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 12699-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12699-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 12699-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 12699-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 12699-1-AP (SGK3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12699-1-AP

  IP result of anti-SGK3 (IP:12699-1-AP, 4ug; Detection:12699-1-AP 1:500) with mouse heart tissue lysate 3200ug.

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  IHC staining of human pancreas cancer using 12699-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12699-1-AP (SGK3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 12699-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 12699-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 12699-1-AP (SGK3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A375 using 12699-1-AP

  Immunofluorescent analysis of A375 cells using 12699-1-AP (SGK3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, humanes Lebergewebe, Maushautgewebe, Rattennierengewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Pankreaskarzinomgewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A375-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

12699-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SGK3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SGK3 fusion protein Ag3149
• Vollständiger Name: serum/glucocorticoid regulated kinase family, member 3
• Berechnetes Molekulargewicht: 496 aa, 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC015326
• Gene symbol: SGK3
• Gene ID (NCBI): 23678
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum- and glucocorticoid-inducible kinase 3 (SGK3) is a protein kinase of the AGC family of protein kinase A, protein kinase G, and protein kinase C and functions downstream of phosphatidylinositol 3-kinase (PI3K)(PMID:21084382). It is also named as DKFZp781N0293, CISK, SGK2, SGKL and may be involved in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. SGK3 mediates cell IL-3-dependent survival signals (PMID:12397388). SGK3 has 2 isoforms produced by alternative splicing with the molecular weight of 57 kDa and 53 kDa.

Publikationen

Species	Application	Title
mouse	WB	Autophagy; Autophagic death of neural stem cells mediates chronic stress-induced decline of adult hippocampal neurogenesis and cognitive deficits.; Authors - Seonghee Jung ; KO Validated
mouse	IF	Front Cell Dev Biol; Single-Cell RNA Sequencing With Combined Use of Bulk RNA Sequencing to Reveal Cell Heterogeneity and Molecular Changes at Acute Stage of Ischemic Stroke in Mouse Cortex Penumbra Area.; Authors - Kang Guo
mouse,rat	WB,IHC,IF	Front Pharmacol; The role of the SGK3/TOPK signaling pathway in the transition from acute kidney injury to chronic kidney disease; Authors - Huapan Shu ; KD Validated