SFRS7 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SFRS7 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Rezensionen (1)

Kat-Nr. : 11044-1-AP

Synonyme

SRSF7, Splicing factor, arginine/serine-rich 7, Serine/arginine-rich splicing factor 7, AAG3

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Galerie der Validierungsdaten

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  WB analysis using 11044-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11044-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 11044-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 11044-1-AP (SFRS7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 11044-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11044-1-AP (SFRS7 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 11044-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11044-1-AP (SFRS7 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 11044-1-AP

  Immunofluorescent analysis of HepG2 cells, using SFRS7 antibody 11044-1-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, HeLa-Zellen, HepG2-Zellen, Maushodengewebe, Rattenhodengewebe, SKOV-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11044-1-AP bindet in WB, IHC, IF/ICC, ELISA SFRS7 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFRS7 fusion protein Ag1526
• Vollständiger Name: splicing factor, arginine/serine-rich 7, 35kDa
• Berechnetes Molekulargewicht: 27 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC000997
• Gene symbol: SFRS7
• Gene ID (NCBI): 6432
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SFRS7 is a member of the serine/arginine (SR) splicing factor family, which mediated the alternative splicing events target the resulting mRNAs for degradation by means of an RNA surveillance pathway called nonsense-mediated mRNA decay. It was found to repress the splicing of MAPT/Tau exon 10

Publikationen

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	WB	Cell; C9orf72 Dipeptide Repeats Impair the Assembly, Dynamics, and Function of Membrane-Less Organelles.; Authors - Kyung-Ha Lee
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan ; KD Validated
human	WB	Nucleic Acids Res; Exonic splicing code and protein binding sites for calcium.; Authors - Reuben J Pengelly ; KD Validated
human	WB	Genomics Proteomics Bioinformatics; Specific Regulation of m6A by SRSF7 Promotes the Progression of Glioblastoma.; Authors - Yixian Cun ; KD Validated
human	WB,IF	Genes Genomics; SRSF7 is a promising prognostic biomarker in hepatocellular carcinoma and is associated with immune infiltration; Authors - Wei Shen ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Jingwen (Verified Customer) (04-28-2021)

The staining signal showed up inside the nucleus.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:150
• Cell Tissue Type: Human cancer cell line