SFRS17A Polyklonaler Antikörper

SFRS17A Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13441-1-AP

Synonyme

CXYorf3, B-lymphocyte antigen, A-kinase anchor protein 17A, AKAP-17A, AKAP17A

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 13441-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13441-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293T using 13441-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 13441-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 13441-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13441-1-AP (SFRS17A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 13441-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13441-1-AP (SFRS17A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 13441-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 13441-1-AP (SFRS17A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 13441-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13441-1-AP (SFRS17A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 13441-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 13441-1-AP (SFRS17A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HT-1080 using 13441-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HT-1080 cells using SFRS17A antibody (13441-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293T-Zellen, humanes Herzgewebe, humanes Nierengewebe, Jurkat-Zellen
• Erfolgreiche Detektion in IHC: Maus-Cerebellum-Gewebe, humanes Pankreaskarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HT-1080.Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13441-1-AP bindet in WB, IHC, IF/ICC, ELISA SFRS17A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFRS17A fusion protein Ag4252
• Vollständiger Name: splicing factor, arginine/serine-rich 17A
• Berechnetes Molekulargewicht: 695 aa, 81 kDa
• Beobachtetes Molekulargewicht: 81 kDa
• GenBank-Zugangsnummer: BC028151
• Gene symbol: SFRS17A
• Gene ID (NCBI): 8227
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.