ASF/SF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ASF/SF2 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Konjugation

Unkonjugiert

Publikationen

30

Rezensionen (2)

Kat-Nr. : 12929-2-AP

Synonyme

SFRS1, pre-mRNA-splicing factor SF2, P33 subunit, OK/SW-cl.3, ASF-1, ASF 1

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Galerie der Validierungsdaten

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  WB analysis using 12929-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12929-2-AP (ASF/SF2 antibody) at dilution of 1:4500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12929-2-AP

  IP result of anti-ASF/SF2 (IP:12929-2-AP, 3ug; Detection:12929-2-AP 1:500) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of human gliomas using 12929-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12929-2-AP (ASF/SF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 12929-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12929-2-AP (ASF/SF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 12929-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ASF/SF2 antibody (12929-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:9000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 26 publications below
• IHC: See 1 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 3 publications below
• RIP: See 4 publications below

Produktinformation

12929-2-AP bindet in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA ASF/SF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ASF/SF2 fusion protein Ag3626
• Vollständiger Name: splicing factor, arginine/serine-rich 1
• Berechnetes Molekulargewicht: 248 aa, 28 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC010264
• Gene symbol: ASF/SF2
• Gene ID (NCBI): 6426
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SFRS1, also named as ASF, SF2, SF2P33, SFRS1 and ASF-1, belongs to the splicing factor SR family. It plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. SFRS1 interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. This antibody can recognize all the 3 isoforms(28kd,33kd and 22kd) of SFRS1.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Zc3h13 Regulates Nuclear RNA m6A Methylation and Mouse Embryonic Stem Cell Self-Renewal.; Authors - Jing Wen
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	J Med Virol; TIM22 and TIM29 inhibit HBV replication by up-regulating SRSF1 expression; Authors - Lin Guo
human	WB	Nucleic Acids Res; Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.; Authors - Misbah Khan
mouse,human	IF,WB	Cell Death Differ; The long non-coding RNA PFI protects against pulmonary fibrosis by interacting with splicing regulator SRSF1.; Authors - Jian Sun
human	WB,IF	Angiogenesis; Extracellular matrix stiffness controls VEGF165 secretion and neuroblastoma angiogenesis via the YAP/RUNX2/SRSF1 axis.; Authors - Min Bao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Katie (Verified Customer) (03-14-2022)

Works well for western blot - clean blot with one band.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000 Western 1:50 IF
• Cell Tissue Type: EL4 cells / Primary Murine CD4 T cells

FH

Yan (Verified Customer) (04-28-2021)

It worked very well when I performed immunofluorescence. Signal was observed in the nucleus.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Human Osteosarcoma Epithelial Cells