SFRP2 Polyklonaler Antikörper

SFRP2 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 12189-1-AP

Synonyme

sFRP-2, Secreted frizzled-related protein 2, Secreted apoptosis-related protein 1, SDF-5, SARP1

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Galerie der Validierungsdaten

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  WB analysis using 12189-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12189-1-AP (SFRP2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of A549 using 12189-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using SFRP2 antibody (12189-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenherzgewebe, Mausherzgewebe
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below

Produktinformation

12189-1-AP bindet in WB, IF/ICC, ELISA SFRP2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SFRP2 fusion protein Ag2853
• Vollständiger Name: secreted frizzled-related protein 2
• Berechnetes Molekulargewicht: 295 aa, 34 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC008666
• Gene symbol: SFRP2
• Gene ID (NCBI): 6423
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Secreted frizzled-related protein 2 (Sfrp2) is a secreted glycoprotein molecule containing an N terminal cysteine-rich domain, which is typically 30-50% identical to the putative Wnt-binding site of the frizzled receptor, and a C terminal heparin-binding domain with weak homology to netrins. It has been implicated in diverse cellular processes, including embryogenesis, regulation of cell apoptosis, and cell differentiation. Methylation of this gene is a potential marker for the presence of colorectal cancer.

Publikationen

Species	Application	Title
human	WB	Cell Res; Comprehensive profiling reveals mechanisms of SOX2-mediated cell fate specification in human ESCs and NPCs.; Authors - Chenlin Zhou
human	WB	Front Oncol; Fucoidan Inhibits the Progression of Hepatocellular Carcinoma via Causing lncRNA LINC00261 Overexpression.; Authors - Danhui Ma
human	WB	Biomed Pharmacother; MicroRNA-500a promotes migration and invasion in hepatocellular carcinoma by activating the Wnt/β-catenin signaling pathway.; Authors - Yuntao Guo
mouse	WB	PLoS One; Digital gene expression tag profiling analysis of the gene expression patterns regulating the early stage of mouse spermatogenesis.; Authors - Zhang Xiujun X
		Physiol Genomics; Tenotomy Immobilization as a Model to Investigate Skeletal Muscle Fibrosis (with Emphasis on Secreted Frizzled-Related Protein 2).; Authors - Uğur Akpulat
mouse	WB	Psychiatry Investig; m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis; Authors - Yue Ming