SF3B3 Polyklonaler Antikörper

SF3B3 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, RIP, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

18

Kat-Nr. : 14577-1-AP

Synonyme

KIAA0017, RSE1, SAP 130, SAP130, SF3b130, SF3B3, Splicing factor 3B subunit 3, STAF130

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Galerie der Validierungsdaten

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  WB analysis using 14577-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 14577-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 14577-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 14577-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 14577-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14577-1-AP (SF3B3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of rat brain using 14577-1-AP

  IP Result of anti-SF3B3 (IP:14577-1-AP, 4ug; Detection:14577-1-AP 1:5000) with rat brain tissue lysate 4800ug.

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  IHC staining of human gliomas using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14577-1-AP (SF3B3 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 14577-1-AP (SF3B3 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14577-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 14577-1-AP (SF3B3 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 14577-1-AP

  Immunofluorescent analysis of Hela cells, using SF3B3 antibody 14577-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  IF Staining of mouse heart using 14577-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse heart using 14577-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using SF3B3 antibody (14577-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, HeLa-Zellen, humanes Hirngewebe, Mausherzgewebe, Rattenherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: Rattenhirngewebe
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Gliomgewebe, Rattenhirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, Mausherzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 15 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Produktinformation

14577-1-AP bindet in WB, RIP, IP, IHC, IF, CoIP, ELISA SF3B3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SF3B3 fusion protein Ag5980
• Vollständiger Name: splicing factor 3b, subunit 3, 130kDa
• Berechnetes Molekulargewicht: 136 kDa
• Beobachtetes Molekulargewicht: 130-135 kDa
• GenBank-Zugangsnummer: BC003146
• Gene symbol: SF3B3
• Gene ID (NCBI): 23450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Introns are removed from nuclear pre-mRNA in 2-step transesterification reactions. Splicing occured in a large ribonucleoprotein particle, called the spliceosome. Spliceosomal intermediate complexes form on pre-mRNA in the order E, A, B, and C, with the catalytic reactions occurring in complex C. U2 small nuclear ribonucleoproteins are one of the proteins essential for spliceosome assembly and mRNA splicing. Functional U2 snRNP is composed of a 12S unit and 2 splicing factors, SF3A, which is composed of 3 proteins, and SF3B, which conposed of 4 proteins. SF3B3 is one of SF3B, and it's required for 'A' complex assembly formed by the stable binding of U2 snRNP to the brachpoint sequence(BPS) in pre-mRNA.

Publikationen

Species	Application	Title
human	WB	Gastroenterology; Enterotoxigenic Bacteroides fragilis promotes intestinal inflammation and malignancy by inhibiting exosomes-packaged miR-149-3p.; Authors - Yingying Cao
		Genome Res; RNA structure replaces the need for U2AF2 in splicing.; Authors - Chien-Ling Lin
human	WB	Nat Commun; Splicing modulators act at the branch point adenosine binding pocket defined by the PHF5A-SF3b complex.; Authors - Teng Teng
human	RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
human	WB	Mol Neurodegener; Nuclear speckle specific hnRNP D-like prevents age- and AD-related cognitive decline by modulating RNA splicing.; Authors - Qingyang Zhang
human	WB	Nucleic Acids Res; Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.; Authors - Ruibing Chen