SESN3 Polyklonaler Antikörper

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• KD/KO Validated

SESN3 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

15

Kat-Nr. : 11431-2-AP

Synonyme

SESN3, SEST3, sestrin 3

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11431-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11431-2-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11431-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 11431-2-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11431-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11431-2-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11431-2-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 11431-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11431-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11431-2-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11431-2-AP (SESN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A431-Zellen, A549-Zellen, HEK-293-Zellen, HepG2-Zellen, Maus-Eierstockgewebe, Mauslebergewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 3 publications below

Produktinformation

11431-2-AP bindet in WB, IHC, ELISA SESN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SESN3 fusion protein Ag1961
• Vollständiger Name: sestrin 3
• Berechnetes Molekulargewicht: 492 aa, 57 kDa
• Beobachtetes Molekulargewicht: 36 kDa, 51-59 kDa
• GenBank-Zugangsnummer: BC017296
• Gene symbol: SESN3
• Gene ID (NCBI): 143686
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 3 was identified as a forkhead box O (FoxO) target gene with antioxidant activity. Recently it was reported that sestrin 3 may play an important role in AKT induced increase in ROS and it might be a promising target in selectively killing cancer cells containing high levels of AKT activity.

Publikationen

Species	Application	Title
human	WB	Dev Cell; FoxOs inhibit mTORC1 and activate Akt by inducing the expression of Sestrin3 and Rictor.; Authors - Chia-Chen Chen ; KD Validated
mouse	IHC	Proc Natl Acad Sci U S A; FOXO1 and FOXO3 transcription factors have unique functions in meniscus development and homeostasis during aging and osteoarthritis.; Authors - Kwang Il Lee
human	WB	Elife; Selective eradication of cancer displaying hyperactive Akt by exploiting the metabolic consequences of Akt activation.; Authors - Veronique Nogueira ; KD Validated
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Exercise ameliorates chronic inflammatory response induced by high-fat diet via Sestrin2 in an Nrf2-dependent manner; Authors - Sujuan Liu
human	WB,IHC	Osteoarthritis Cartilage; Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.; Authors - T Shen
mouse	IHC	J Ovarian Res; Sestrin1, 2, and 3 are dispensable for female fertility in mice; Authors - Mengchen Wang