SERPING1/C1 Inactivator Polyklonaler Antikörper

SERPING1/C1 Inactivator Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 12259-1-AP

Synonyme

Serpin G1, SERPING1, C1NH, C1-inhibiting factor, C1IN

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Galerie der Validierungsdaten

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  WB analysis using 12259-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12259-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 12259-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 12259-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12259-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12259-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12259-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12259-1-AP (SERPING1/C1 Inactivator antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 12259-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12259-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12259-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SERPING1/C1 Inactivator antibody (12259-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 12259-1-AP

  Immunofluorescent analysis of HepG2 cells using 12259-1-AP (SERPING1/C1 Inactivator antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma, HeLa-Zellen, HepG2-Zellen, humanes Lebergewebe, humanes Uringewebe, L02-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Produktinformation

12259-1-AP bindet in WB, IHC, IF/ICC, ELISA SERPING1/C1 Inactivator und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERPING1/C1 Inactivator fusion protein Ag2819
• Vollständiger Name: serpin peptidase inhibitor, clade G (C1 inhibitor), member 1
• Berechnetes Molekulargewicht: 500 aa, 55 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC011171
• Gene symbol: SERPING1
• Gene ID (NCBI): 710
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein. Of its 95-105 kDa apparent molecular mass, the protein moiety of 500 aa accounts for only 55 kDa. The native 105 kDa form of C1INH could be cleaved into 60-65 kDa fragments.

Publikationen

Species	Application	Title
mouse	WB	Glia; Knockdown of circulating C1 inhibitor induces neurovascular impairment, glial cell activation, neuroinflammation, and behavioral deficits.; Authors - Dorit Farfara
mouse	IF	J Neuroinflammation; Fluoxetine inhibited the activation of A1 reactive astrocyte in a mouse model of major depressive disorder through astrocytic 5-HT2BR/β-arrestin2 pathway.; Authors - Yinquan Fang
human	WB	J Thromb Haemost; The Alzheimer's disease peptide Aβ promotes thrombin generation through activation of coagulation factor XII.; Authors - Daria Zamolodchikov
mouse	WB	J Thromb Haemost; The Alzheimer's disease peptide β-amyloid promotes thrombin generation through activation of coagulation factor XII: reply.; Authors - D Zamolodchikov
human	IHC	J Alzheimers Dis; A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology.; Authors - Koji Abe
human	IHC	Urol Oncol; Decreased expression of serine protease inhibitor family G1 (SERPING1) in prostate cancer can help distinguish high-risk prostate cancer and predicts malignant progression.; Authors - Shengmeng Peng