SerpinB3/SerpinB4 Polyklonaler Antikörper

SerpinB3/SerpinB4 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11428-1-AP

Synonyme

LEUPIN, Peptidase inhibitor 11, PI 11, PI11, SCCA, SCCA 2, SCCA1, SCCA2, Serpin B4, SERPINB3, SERPINB4

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Galerie der Validierungsdaten

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  WB analysis using 11428-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11428-1-AP (SERPINB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 11428-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 11428-1-AP (SERPINB4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, COLO 320-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

11428-1-AP bindet in WB, ELISA SerpinB3/SerpinB4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SerpinB3/SerpinB4 fusion protein Ag1964
• Vollständiger Name: serpin peptidase inhibitor, clade B (ovalbumin), member 4
• Berechnetes Molekulargewicht: 390 aa, 45 kDa
• Beobachtetes Molekulargewicht: 42-45 kDa
• GenBank-Zugangsnummer: BC017401
• Gene symbol: SERPINB4
• Gene ID (NCBI): 6318
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Squamous cell carcinoma antigens 1 and 2 (SCCA1 and 2, SERPIN B3 and B4) are members of the ovalbumin family of serine proteinase inhibitors. SCCA1 and SCCA2 are highly homologous proteins, 91% identical at the amino acid level, and probably evolving from a common ancestor gene. The neutral form of SCCA (SCCA1, or SERPINB3) is detected in the cytoplasm of normal and some malignant squamous cells, whereas the acidic form (SCCA2, or SERPINB4) is expressed primarily in malignant cells and is the major form found in the plasma of cancer patients. SCCA2 (SERPINB4), along with SCCA1(SERPINB3), can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation. This antibody can recognize both SerpinB3 and SerpinB4.