SERPINA6/CBG Polyklonaler Antikörper

SERPINA6/CBG Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen

1

Kat-Nr. : 12010-1-AP

Synonyme

SERPINA6, Serpin A6, CBG, Transcortin, Corticosteroid-binding globulin

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Galerie der Validierungsdaten

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  WB analysis of human plasma using 12010-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12010-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 12010-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 12010-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12010-1-AP (SERPINA6 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12010-1-AP (SERPINA6 antibody at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver cancer using 12010-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 12010-1-AP (SERPINA6 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 12010-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 12010-1-AP (SERPINA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Plasmagewebe, A375-Zellen, HEK-293-Zellen, L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Lebergewebe, humanes Leberkarzinomgewebe, humanes Leberzirrhosegewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

12010-1-AP bindet in WB, IHC, IF/ICC, ELISA SERPINA6/CBG und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SERPINA6/CBG fusion protein Ag2623
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6
• Berechnetes Molekulargewicht: 405 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC058021
• Gene symbol: SERPINA6
• Gene ID (NCBI): 866
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINA6, also known as corticosteroid-binding globulin or CBG, belongs to the serpin family of serine protease inhibitors, and is mainly secreted by the liver. It is an alpha-globulin protein with corticosteroid-binding properties, and involved in obesity and stress sensitivity. It is also the major transport protein for glucorticoids and progestins in the blood of most vertebrates. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency).

Publikationen

Species	Application	Title
human	WB	PLoS One; Quantitative mass spectrometry analysis using PAcIFIC for the identification of plasma diagnostic biomarkers for abdominal aortic aneurysm.; Authors - Acosta-Martin Adelina E AE