Protein C inhibitor Monoklonaler Antikörper

Protein C inhibitor Monoklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H6G2

Kat-Nr. : 66030-1-Ig

Synonyme

PAI3, PCI, PLANH3, PROCI, Protein C inhibitor, SERPINA5

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Galerie der Validierungsdaten

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  WB analysis of human testis using 66030-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 66030-1-Ig

  human testis tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 66030-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 66030-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 66030-1-Ig (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 66030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 66030-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66030-1-Ig (Protein C inhibitor antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human ovary tumor using 66030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  IF Staining of human ovary tumor using 66030-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human ovary tumor tissue using Protein C inhibitor antibody (66030-1-Ig, Clone: 2H6G2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Human testis, Gewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Ovarialkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

66030-1-Ig bindet in WB, IHC, IF, ELISA Protein C inhibitor und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Protein C inhibitor fusion protein Ag18094
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC008915
• Gene symbol: SERPINA5
• Gene ID (NCBI): 5104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.