Protein C inhibitor Polyklonaler Antikörper

Protein C inhibitor Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Kat-Nr. : 10673-1-AP

Synonyme

Serpin A5, PROCI, Plasminogen activator inhibitor 3, Plasma serine protease inhibitor, PLANH3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 10673-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10673-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 10673-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10673-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 10673-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10673-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10673-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 10673-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10673-1-AP (Protein C inhibitor antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 10673-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 10673-1-AP (Protein C inhibitor antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, HeLa-Zellen, Jurkat-Zellen, L02-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe, humanes Hodengewebe, humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:600
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 5 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

10673-1-AP bindet in WB, IHC, IF/ICC, CoIP, ELISA Protein C inhibitor und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Protein C inhibitor fusion protein Ag1078
• Vollständiger Name: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 5
• Berechnetes Molekulargewicht: 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC008915
• Gene symbol: SERPINA5
• Gene ID (NCBI): 5104
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SERPINA5 (also called Protein C Inhibitor, PCI ) is a serpin type of serine protease inhibitor that is found in many tissues and fluids in human, including blood plasma, seminal plasma and urine. PCI found in blood originates from the liver and is capable of inhibiting several serine proteases involved in the regulation of coagulation and fibrinolysis, including activated protein C, thrombin, factor Xa, various kallikreins and plasminogen activators. PCI has been found to have antimicrobial and antitumor properties and thus appears to be a multi-functional protein.

Publikationen

Species	Application	Title
human	IHC	Mod Pathol; Loss of SerpinA5 protein expression is associated with advanced-stage serous ovarian tumors.; Authors - Bijsmans Ingrid T G W IT
human	IHC	J Neuropathol Exp Neurol; Gene expression profiling of NF-1-associated and sporadic pilocytic astrocytoma identifies aldehyde dehydrogenase 1 family member L1 (ALDH1L1) as an underexpressed candidate biomarker in aggressive subtypes.; Authors - Rodriguez Fausto J FJ
rat	IHC	Proteomics Clin Appl; Proteomic analysis of human articular cartilage unravels the dyscoagulation in osteoarthritis and the potential value of serpinA5 as a biomarker for osteoarthritis.; Authors - Tengjiao Zhu
human	WB,CoIP	J Cell Mol Med; SERPINA5 promotes tumour cell proliferation by modulating the PI3K/AKT/mTOR signalling pathway in gastric cancer; Authors - Meiyang Fan
rat	ELISA,IHC	Biomolecules; Improvement in Clinical Features of L-NAME-Induced Preeclampsia-like Rats through Reduced SERPINA5 Expression; Authors - Shanshui Zeng
human,rat	IHC	J Proteome Res; Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis; Authors - Qizhao Tan