Septin 4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Septin 4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 12476-1-AP

Synonyme

SEPT4, hucep7, hCDCREL-2, hCDCREL2, Cell division control-related protein 2

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Galerie der Validierungsdaten

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  WB analysis using 12476-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12476-1-AP (Septin 4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 12476-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 12476-1-AP (Septin 4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A375 using 12476-1-AP

  IP result of anti-Septin 4 (IP:12476-1-AP, 4ug; Detection:12476-1-AP 1:300) with A375 cells lysate 2200ug.

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  IHC staining of mouse brain using 12476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12476-1-AP (Septin 4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12476-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12476-1-AP (Septin 4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of hTERT-RPE1 using 12476-1-AP

  Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Septin 4 antibody (12476-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A375-Zellen, Maus-Cerebellum-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: A375-Zellen
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: hTERT-RPE1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

12476-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA Septin 4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Septin 4 fusion protein Ag3185
• Vollständiger Name: septin 4
• Berechnetes Molekulargewicht: 459 aa, 53 kDa
• Beobachtetes Molekulargewicht: 54 kDa, 52 kDa, 48 kDa, 44 kDa
• GenBank-Zugangsnummer: BC018056
• Gene symbol: SEPT4
• Gene ID (NCBI): 5414
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spetin 4 is a member of the septin family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Spetin 4 was widely expressed in brain and heart, also expressed in colorectal cancers and malignant melanomas. This protein has been implicated in diverse cellular functions, including cytokinesis, apoptosis, and tumor suppression(PMID:15737931). There are several isoforms of SEPTIN4, four of which were detected in the mouse brain and mouse cerebellum, 54 kDa, 52 kDa, 48 kDa and 44 kDa respectively(PMID: 23938054; PMID: 26657011).

Publikationen

Species	Application	Title
human	WB	Bioorg Chem; Discovery of a septin-4 covalent binder with antimetastatic activity in a mouse model of melanoma; Authors - Federica Blua
human	WB	Int J Biol Sci; Septin4 Prevents PDGF-BB-induced HAVSMC Phenotypic Transformation, Proliferation and Migration by Promoting SIRT1-STAT3 Deacetylation and Dephosphorylation.; Authors - Naijin Zhang ; KD Validated
human	WB,IF,IP	Biochem Biophys Res Commun; Septin4 as a novel binding partner of PARP1 contributes to oxidative stress induced human umbilical vein endothelial cells injure.; Authors - Naijin Zhang ; KD Validated
human,mouse	IF,CoIP	Front Endocrinol (Lausanne); A novel mutation in CFAP47 causes male infertility due to multiple morphological abnormalities of the sperm flagella; Authors - Mohan Liu
mouse	WB	Biomed Pharmacother; Atorvastatin rescues vascular endothelial injury in hypertension by WWP2-mediated ubiquitination and degradation of ATP5A; Authors - Zeyu Yin

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Mohammed (Verified Customer) (08-19-2020)

Septin 4 stained the annulus of sperm very beautifully. Very little background and a nice sharp stain.

• Applications: Immunofluorescence
• Primary Antibody Dilution: (1:200)
• Cell Tissue Type: Testis