SEC63 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SEC63 Polyklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 13978-1-AP

Synonyme

Translocation protein SEC63 homolog, SEC63L, DNAJC23, DnaJ homolog subfamily C member 23

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Galerie der Validierungsdaten

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  WB analysis of A431 using 13978-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13978-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13978-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13978-1-AP (SEC63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 13978-1-AP

  IP result of anti-SEC63 (IP:13978-1-AP, 4ug; Detection:13978-1-AP 1:300) with A431 cells lysate 2400ug.

• 

  IHC staining of human liver using 13978-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 13978-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 13978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 13978-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 13978-1-AP (SEC63 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 13978-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 13978-1-AP (SEC63 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse kidney using 13978-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using 13978-1-AP (SEC63 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HEK-293 using 13978-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using SEC63 antibody (13978-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, HEK-293-Zellen, PC-3-Zellen
• Erfolgreiche IP: A431-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, Maus-Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Mausnierengewebe
• Erfolgreiche Detektion in IF/ICC: HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IF: See 1 publications below

Produktinformation

13978-1-AP bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA SEC63 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SEC63 fusion protein Ag5021
• Vollständiger Name: SEC63 homolog (S. cerevisiae)
• Berechnetes Molekulargewicht: 88 kDa
• Beobachtetes Molekulargewicht: 83-90 kDa
• GenBank-Zugangsnummer: BC047221
• Gene symbol: SEC63
• Gene ID (NCBI): 11231
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC63 is an integral ER membrane protein. SEC63 and SEC62 form a dimeric complex and play a central role in the translocation of nascent and newly synthesized precursor polypeptides into the ER. Mutations in the gene encoding SEC63 cause polycystic liver disease in humans. The intact SEC63 protein is usually detected around 83-90 kDa, while smaller cleavage products can be observed by autophagy proteolysis (PMID: 25052096)

Publikationen

Species	Application	Title
	WB	Mol Cell; Cotranslational Stabilization of Sec62/63 within the ER Sec61 Translocon Is Controlled by Distinct Substrate-Driven Translocation Events.; Authors - Brian J Conti
mouse	WB	J Clin Invest; Sec63 and Xbp1 regulate IRE1α activity and polycystic disease severity.; Authors - Sorin V Fedeles
mouse	WB, IF	J Am Soc Nephrol; Essential Role of X-Box Binding Protein-1 during Endoplasmic Reticulum Stress in Podocytes.; Authors - Hossam Hassan ; KO Validated
human	WB	J Biol Chem; ER entry pathway and glycosylation of GPI-anchored proteins are determined by N-terminal signal sequence and C-terminal GPI-attachment sequence; Authors - Tetsuya Hirata ; KO Validated