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SEC24D Monoklonaler Antikörper

SEC24D Monoklonal Antikörper für IF/ICC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

IF/ICC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

3G5G9

Kat-Nr. : CL594-67409

Synonyme

SEC24-related protein D, SEC24 related protein D, KIAA0755, 3G5G9

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated SEC24D Monoclonal antibody (CL594-67409)

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:100. DAPI (blue).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated SEC24D Monoclonal antibody (CL594-67409)

    IF Staining of HepG2 using CL594-67409

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 SEC24D antibody (CL594-67409, Clone: 3G5G9 ) at dilution of 1:200.

Geprüfte Anwendungen

Erfolgreiche Detektion in IF/ICCHepG2-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-67409 bindet in IF/ICC SEC24D und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG2a
Klonalität Monoklonal
Typ Antikörper
Immunogen SEC24D fusion protein Ag4657
Vollständiger Name SEC24 family, member D (S. cerevisiae)
Berechnetes Molekulargewicht 1033 aa, 113 kDa
Beobachtetes Molekulargewicht 113 kDa
GenBank-ZugangsnummerBC035761
Gene symbol SEC24D
Gene ID (NCBI) 9871
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

COPII-coated vesicles mediate anterograde transport from the ER to the Golgi apparatus. COPII is composed of three parts: two coat protein complexes (Sec23/24 complex and Sec13/31 complex) and one small GTP-binding protein, Sar1 (PMID: 9568718). Sec24 subunit of the coat complex has been implicated in cargo recognition (PMID: 17316621). In mammal cells there are four Sec24 isoforms, termed Sec24A, Sec24B, Sec24C, and Sec24D (PMID: 10329445).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
IF protocol for CL594 SEC24D antibody CL594-67409Protokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen