SEC11A Monoklonaler Antikörper

SEC11A Monoklonal Antikörper für IHC, WB,ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC,ELISA

Konjugation

Unkonjugiert

CloneNo.

2B11G12

Kat-Nr. : 67379-1-Ig

Synonyme

1810012E07Rik, Endopeptidase SP18, SEC11 homolog A, SEC11 like protein 1, SEC11A, SEC11L1, sid2895, SPase 18 kDa subunit, SPC18, SPCS4A

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Galerie der Validierungsdaten

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  WB analysis using 67379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67379-1-Ig (SEC11A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67379-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67379-1-Ig (SEC11A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 67379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67379-1-Ig (SEC11A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 67379-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67379-1-Ig (SEC11A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, 4T1-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67379-1-Ig bindet in WB, IHC,ELISA SEC11A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SEC11A fusion protein Ag6610
• Vollständiger Name: SEC11 homolog A (S. cerevisiae)
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 17-21 kDa
• GenBank-Zugangsnummer: BC000359
• Gene symbol: SEC11A
• Gene ID (NCBI): 23478
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SEC11A(Signal peptidase complex catalytic subunit SEC11A) is also named as SEC11L1, SPC18, SPCS4A and belongs to the peptidase S26B family. It is a component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum. SEC11A has some isoforms with the molecular mass of 17-21 kDa.