SDPR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

SDPR Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Kat-Nr. : 12339-1-AP

Synonyme

PS-p68, PS p68, Phosphatidylserine-binding protein, Cavin-2, Cavin 2

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Galerie der Validierungsdaten

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  WB analysis of A549 using 12339-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12339-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 12339-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12339-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12339-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 12339-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse placenta using 12339-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12339-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12339-1-AP (SDPR Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 12339-1-AP

  IP result of anti-SDPR (IP:12339-1-AP, 4ug; Detection:12339-1-AP 1:1000) with mouse heart tissue lysate 8000ug.

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  IHC staining of human renal cell carcinoma using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human kidney using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast hyperplasia using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human breast hyperplasia using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast hyperplasia tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human renal cell carcinoma using 12339-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 12339-1-AP (SDPR Antibody) at dilution of 1:200 (under 10x lens).

• 

  IF Staining of A549 using 12339-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 12339-1-AP (SDPR antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, COLO 320-Zellen, Mausherzgewebe, Maushirngewebe, Mauslebergewebe, Maus-Plazenta-Gewebe, Rattenhirngewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Nierenzellkarzinomgewebe, humanes Herzgewebe, humanes Mammahyperplasie-Gewebe, humanes Nierengewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 12 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

12339-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SDPR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SDPR fusion protein Ag2990
• Vollständiger Name: serum deprivation response (phosphatidylserine binding protein)
• Berechnetes Molekulargewicht: 425 aa, 47 kDa
• Beobachtetes Molekulargewicht: 68-70 kDa
• GenBank-Zugangsnummer: BC016475
• Gene symbol: SDPR
• Gene ID (NCBI): 8436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Serum deprivation-response protein(SDRP), a substrate of protein kinase C, is a member of a group of proteins that bind to phosphatidylserine in a clacium-dependent manner. SDPR may participate in the generation or stabilization of membrane curvature. SDPR is highly expressed in heart and lung, and low in brain,kidney, liver, pancreas, placenta and skeletal muscle. This is a rabbit polyclonal anti-SDPR antibody raised against C-terminus of human SDPR. SDRP of molecular masses 115 kDa and 100 kDa were found in neutrophils, monocytes, lymphocytes, platelets and erythrocytes. In platelets, however, the principal SDRP is a cytosolic protein of molecular mass 68 kDa. (PMID: 2390065 )

Publikationen

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao ; KD Validated
human	IF	Dev Cell; Cellular heterogeneity of human fallopian tubes in normal and hydrosalpinx disease states identified using scRNA-seq.; Authors - Nicole D Ulrich
mouse	WB	Haematologica; Dynamin 2 is required for GPVI signaling and platelet hemostatic function in mice.; Authors - Nathan Eaton
mouse	WB,IF,IP	Mol Metab; Requirement of Cavin-2 for the expression and stability of IRβ in adequate adipocyte differentiation.; Authors - Yusuke Higuchi
rat	IF	J Cereb Blood Flow Metab; P-glycoprotein traffics from the nucleus to the plasma membrane in rat brain endothelium during inflammatory pain.; Authors - Margaret E Tome
human	WB,IHC	Cancer Cell Int; Prognostic values, ceRNA network, and immune regulation function of SDPR in KRAS-mutant lung cancer.; Authors - Xiaoqing Luo