SDCCAG1 Polyklonaler Antikörper

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• KD/KO Validated

SDCCAG1 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

8

Kat-Nr. : 11840-1-AP

Synonyme

NEMF, NY CO 1, SDCCAG1

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Galerie der Validierungsdaten

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  WB analysis using 11840-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11840-1-AP (SDCCAG1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 11840-1-AP

  IP result of anti-SDCCAG1 (IP:11840-1-AP, 4ug; Detection:11840-1-AP 1:500) with A549 cells lysate 1800ug.

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  IHC staining of human lung cancer using 11840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11840-1-AP (SDCCAG1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 11840-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11840-1-AP (SDCCAG1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Jurkat-Zellen
• Erfolgreiche IP: A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Produktinformation

11840-1-AP bindet in WB, IHC, IF, IP, ELISA SDCCAG1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SDCCAG1 fusion protein Ag2467
• Vollständiger Name: serologically defined colon cancer antigen 1
• Berechnetes Molekulargewicht: 1076 aa, 123 kDa
• Beobachtetes Molekulargewicht: 130-140 kDa
• GenBank-Zugangsnummer: BC020794
• Gene symbol: SDCCAG1
• Gene ID (NCBI): 9147
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDCCAG1, also named as NEMF, is a nuclear export mediator. It can be bound to translocon-engaged 60S subunits on native ER membranes. Human SDCCAG1 has been implicated in lung cancers and it may function as a tumor suppressor gene. SDCCAG1 inactivation contributes to the transformed state of human lung cancer cells (PMID:16103875, 10575219). NEMF is also regarded as a potential biomarker for the detection of active and latent TB infections (PMID: 26818387).

Publikationen

Species	Application	Title
human	WB	Nature; UFM1 E3 ligase promotes recycling of 60S ribosomal subunits from the ER; Authors - Paul A DaRosa
	WB	Mol Cell; Convergence of mammalian RQC and C-end rule proteolytic pathways via alanine tailing.; Authors - Anna Thrun ; KO Validated
mouse	WB	Nat Commun; NEMF mutations that impair ribosome-associated quality control are associated with neuromuscular disease.; Authors - Paige B Martin ; KO Validated
human	WB	Cell Rep; NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP; Authors - Liang Lv
human	WB,IF	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human	WB	bioRxiv; Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats; Authors - Yi-Ju Tseng