Syntenin 2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

Syntenin 2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

5

Rezensionen (1)

Kat-Nr. : 10407-1-AP

Synonyme

SDCBP2, SITAC18, ST 2, Syndecan binding protein 2, Syntenin 2

---

Galerie der Validierungsdaten

• 

  WB analysis of mouse brain using 10407-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10407-1-AP (Syntenin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10407-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10407-1-AP (Syntenin 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 10407-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 10407-1-AP (Syntenin 2 antibody) at dilution of 1:250 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10407-1-AP (Syntenin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10407-1-AP (Syntenin 2 antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human gliomas using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10407-1-AP (Syntenin 2 antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10407-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10407-1-AP (Syntenin 2 antibody at dilution of 1:50 (under 40x lens).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, humanes Lebergewebe, Mausherzgewebe
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

10407-1-AP bindet in WB, IF, IHC, CoIP, ELISA Syntenin 2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syntenin 2 fusion protein Ag0668
• Vollständiger Name: syndecan binding protein (syntenin) 2
• Berechnetes Molekulargewicht: 32 kDa
• Beobachtetes Molekulargewicht: 35-37 kDa
• GenBank-Zugangsnummer: BC002727
• Gene symbol: Syntenin 2
• Gene ID (NCBI): 27111
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syndecan-binding protein 2 (SDCBP2, Syntenin 2) is a protein containing two PDZ domains. PDZ domains facilitate protein-protein interactions by binding to the cytoplasmic C-terminus of transmembrane proteins. SDCBP2 binds with high affinity to PIP2 and is targeted to the plasma membrane, nucleoli and nuclear speckles via its PDZ domains (PMID: 15961997). SDCBP2 exists in two alternatively spliced variants, 2α and 2β, which differ in the lengths of their N-terminal regions. (PMID: 15276154)

Publikationen

Species	Application	Title
human	WB,IHC	J Virol; Human papillomavirus type 8 E6 oncoprotein inhibits transcription of the PDZ protein syntenin-2.; Authors - Lazi? Daliborka D
human,mouse	WB,IHC,CoIP	Cell Signal; Targeting SDCBP2 in acute myeloid leukemia; Authors - Yan Du ; KD Validated
human	WB, IHC, IF	Front Microbiol; HPV8-E6 Interferes with Syntenin-2 Expression through Deregulation of Differentiation, Methylation and Phosphatidylinositide-Kinase Dependent Mechanisms.; Authors - Benjamin Marx ; KD Validated
human,mouse	WB	Front Neurosci; MiR-409-5p as a Regulator of Neurite Growth Is Down Regulated in APP/PS1 Murine Model of Alzheimer's Disease.; Authors - Jing Guo ; KD Validated
mouse	IF	Gut Pathog; Virulence factors and mechanisms of paediatric pneumonia caused by Enterococcus faecalis; Authors - Zhiying Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver tissue lysates were subjected to SDS PAGE followed by western blot with this antibody at dilution of 1:2000 incubated at 4 degree overnight. Syntenin 2 appeared at 36 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse liver tissue