SDC4 Polyklonaler Antikörper

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• KD/KO Validated

SDC4 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 11820-1-AP

Synonyme

Amphiglycan, Ryudocan core protein, SDC4, SYND4, syndecan 4

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Galerie der Validierungsdaten

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  WB analysis using 11820-1-AP

  NIH/3T3 lysates were subjected to SDS PAGE followed by western blot with 11820-1-AP (SDC4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11820-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11820-1-AP (SDC4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Produktinformation

11820-1-AP bindet in WB, IF, IHC, ELISA SDC4 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SDC4 fusion protein Ag2388
• Vollständiger Name: syndecan 4
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22-26 kDa
• GenBank-Zugangsnummer: BC030805
• Gene symbol: SDC4
• Gene ID (NCBI): 6385
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SDC4, also named as syndecan 4, is a cell surface proteoglycan that bears heparan sulfate, which is involved in normal wound healing. It has been reported that SDC4may be associated with keratinocyte proliferation and differentiation in the epidermis. SDC4 also plays a key role in the angiogenesis and inflammation. The predicted MW of SDC4 is 22 kDa and 11820-1-AP antibody recognizes a protein around 24-26 kDa and 48-52 kDa (homodimer) which is similar to the papers.(PMID: 20639201, 21949132; 18638267; 30165731 )

Publikationen

Species	Application	Title
rat	WB,IF	Front Pharmacol; Dioscin Inhibits HSC-T6 Cell Migration via Adjusting SDC-4 Expression: Insights from iTRAQ-Based Quantitative Proteomics.; Authors - Lianhong Yin
human	WB	Oncol Lett; Cadherin-16 inhibits thyroid carcinoma cell proliferation and invasion.; Authors - Xiaolin Yang
human	WB,IF,IHC	Cell Death Dis; Therapeutic potential of targeting membrane-spanning proteoglycan SDC4 in hepatocellular carcinoma.; Authors - Heng Yang ; KD Validated
human	WB	eBioMedicine; Atypical E3 ligase ZFP91 promotes small-molecule-induced E2F2 transcription factor degradation for cancer therapy; Authors - Ting-Ting Liu
mouse	IHC,IF	Front Pediatr; Bioinformatics analysis identifies heparan sulfate proteoglycans acting as different progress subtypes of biliary atresia; Authors - Zequan Ding
human	IHC	EBioMedicine; Spatial and single-cell colocalisation analysis reveals MDK-mediated immunosuppressive environment with regulatory T cells in colorectal carcinogenesis; Authors - Masahiro Hashimoto