Syndecan-3 Polyklonaler Antikörper

Syndecan-3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (2)

Kat-Nr. : 10886-1-AP

Synonyme

SDC3, syndecan 3, SYND3, SDCN, N syndecan

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Galerie der Validierungsdaten

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  WB analysis using 10886-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10886-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 10886-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10886-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10886-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10886-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 10886-1-AP

  IP result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:1000) with mouse lung tissue lysate 4000ug.

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  IP experiment of A549 using 10886-1-AP

  IP result of anti-Syndecan-3 (IP:10886-1-AP, 4ug; Detection:10886-1-AP 1:600) with A549 cells lysate 2800ug.

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  IHC staining of human colon cancer using 10886-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10886-1-AP (Syndecan-3 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10886-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10886-1-AP (Syndecan-3 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 10886-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using Syndecan-3 antibody (10886-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 10886-1-AP

  Immunofluorescent analysis of MCF-7 cells, using SDC3 antibody 10886-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, Jurkat-Zellen, L02-Zellen, Mauslungengewebe, Mausnierengewebe, MCF-7-Zellen, Rattenlungengewebe, Rattennierengewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Mauslungengewebe, A549-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen, MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Produktinformation

10886-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA Syndecan-3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Syndecan-3 fusion protein Ag1317
• Vollständiger Name: syndecan 3
• Berechnetes Molekulargewicht: 38 kDa
• Beobachtetes Molekulargewicht: 60-70 kDa
• GenBank-Zugangsnummer: BC013974
• Gene symbol: Syndecan-3
• Gene ID (NCBI): 9672
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Syndecan-3 is a member o f the Syndecan proteoglycan family. It plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism.

Publikationen

Species	Application	Title
human	IHC	Am J Pathol; Screening for potential targets for therapy in mesenchymal, clear cell, and dedifferentiated chondrosarcoma reveals Bcl-2 family members and TGFβ as potential targets.; Authors - van Oosterwijk Jolieke G JG
human	IHC	Am J Pathol; No haploinsufficiency but loss of heterozygosity for EXT in multiple osteochondromas.; Authors - Reijnders Christianne M A CM
human	WB	J Cell Sci; Electrophoresis of cell membrane heparan sulfate regulates galvanotaxis in glial cells.; Authors - Yu-Ja Huang
human	IHC	BMC Cancer; Prognostic significance of the expression of GFRα1, GFRα3 and syndecan-3, proteins binding ARTEMIN, in mammary carcinoma.; Authors - Wu Zheng-Sheng ZS
human	IHC	Medicine (Baltimore); Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3; Authors - Guozhi Yang
mouse	IF	Int J Mol Sci; GDNF Increases Inhibitory Synaptic Drive on Principal Neurons in the Hippocampus via Activation of the Ret Pathway; Authors - Apostolos Mikroulis

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kamal (Verified Customer) (02-15-2024)

Mouse liver lysates were subjected to SDS PAGE followed by western blot with 10886-1-AP (Syndecan-3 antibody) at dilution of 1:2000 in 1X TBST incubated overnight at 4 degree C. Syndecan-3 appeared at 65 kDa.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: WESTERN BLOT (1:2000), IMMUNOFLUORESCENCE (1:100)
• Cell Tissue Type: Mouse liver tissue

FH

Ryan (Verified Customer) (02-28-2019)

Tissue was fixed in PFA with tris-HCl antigen retrieval ph=6. Co-localisation with microglia based on known markers (not shown).

• Applications: Immunohistochemistry, Immunofluorescence,
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Zebrafish retina (Microglia) at 3dpf

Syndecan-3 Antibody Immunohistochemistry,Immunofluorescence, validation (1:100 dilution) in Zebrafish retina (Microglia) at 3dpf (Cat no:10886-1-AP)