EMAP II Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

EMAP II Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 11091-1-AP

Synonyme

SCYE1, Endothelial monocyte-activating polypeptide 2, EMAPII, EMAP2, EMAP 2

---

Galerie der Validierungsdaten

• 

  WB analysis of A549 using 11091-1-AP

  WB result of EMAP II antibody (11091-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EMAP II transfected A549 cells.

• 

  WB analysis using 11091-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11091-1-AP (EMAP II antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SGC-7901 using 11091-1-AP

  IP result of anti-EMAP II (IP:11091-1-AP, 4ug; Detection:11091-1-AP 1:500) with SGC-7901 cells lysate 2200 ug.

• 

  IHC staining of human cervical cancer using 11091-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 11091-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11091-1-AP (EMAP II antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11091-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EMAP II antibody (11091-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Maushirngewebe, SGC-7901-Zellen, SH-SY5Y-Zellen, U-251-Zellen
• Erfolgreiche IP: SGC-7901-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

11091-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA EMAP II und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: EMAP II fusion protein Ag1560
• Vollständiger Name: small inducible cytokine subfamily E, member 1 (endothelial monocyte-activating)
• Berechnetes Molekulargewicht: 34 kDa
• Beobachtetes Molekulargewicht: 34 kDa and 68 kDa
• GenBank-Zugangsnummer: BC014051
• Gene symbol: EMAP II
• Gene ID (NCBI): 9255
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCYE1(small inducible cytokine subfamily E, member 1) is also named as EMAP2, AIMP1. The human cDNA encodes a 312-amino acid polypeptide that the predicted 34-kDa precursor can be cleaved to produce an active 20-kDa product(PMID:7929199). SCYE1 is secreted as a result of various stimuli, including TNF-α, heat shock, and hypoxia, and that it acts as a multifunctional cytokine on both endothelial and immune cells(PMID:18292511). Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). It has 2 isoforms produced by alternative splicing and the protein can exsit as a dimer(PMID:11306575).

Publikationen

Species	Application	Title
human	WB	Cancer Lett; Trim21-mediated HIF-1α degradation attenuates aerobic glycolysis to inhibit renal cancer tumorigenesis and metastasis.; Authors - Xintian Chen
human	WB	J Biol Chem; Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains.; Authors - Dalia Halawani
human	WB	Proteomics; Mass Spectrometric Analysis Identifies AIMP1 and LTA4H as FSCN1-Binding Proteins in Laryngeal Squamous Cell Carcinoma.; Authors - Wei Gao
rat	IF	Int J Mol Med; Changes in the expression of endothelial monocyte‑activating polypeptide II in the rat hippocampus following status epilepticus.; Authors - Chun Li
human	IHC,WB	Cancer Commun (Lond); AIMP1 promotes multiple myeloma malignancy through interacting with ANP32A to mediate histone H3 acetylation; Authors - Rongfang Wei
human	WB	Proc Natl Acad Sci U S A; Multimodal cotranslational interactions direct assembly of the human multi-tRNA synthetase complex; Authors - Krishnendu Khan ; KD Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Krishnendu (Verified Customer) (02-27-2020)

Very strong antibody and works nicely for both WB and IP.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1:2000 for WB and 1:100 for IP
• Cell Tissue Type: HEK293T