SCRN3 Polyklonaler Antikörper

SCRN3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Rezensionen (1)

Kat-Nr. : 13240-1-AP

Synonyme

SES3, Secernin-3, secernin 3

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Galerie der Validierungsdaten

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  WB analysis of HepG2 using 13240-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 13240-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 13240-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 13240-1-AP (SCRN3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13240-1-AP

  IP result of anti-SCRN3 (IP:13240-1-AP, 4ug; Detection:13240-1-AP 1:800) with HEK-293 cells lysate 1760ug.

• 

  IHC staining of human pancreas using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human pancreas using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 13240-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13240-1-AP (SCRN3 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 13240-1-AP

  Immunofluorescent analysis of HepG2 cells using 13240-1-AP (SCRN3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, A549-Zellen, HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Pankreasgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

13240-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SCRN3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SCRN3 fusion protein Ag3777
• Vollständiger Name: secernin 3
• Berechnetes Molekulargewicht: 424 aa, 49 kDa
• Beobachtetes Molekulargewicht: 49 kDa
• GenBank-Zugangsnummer: BC031821
• Gene symbol: SCRN3
• Gene ID (NCBI): 79634
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kwan Ho (Verified Customer) (05-13-2022)

This Ab can detect endogenous SCRN3 in HEK293T cells, which was validated with KO cells. However, it also binds to off-targets.

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T cells